Essential role of neutrophils but not mammary alveolar macrophages in a murine model of acute Escherichia coli mastitis
| Autor: | Sharon Elazar, Nahum Y. Shpigel, Ilan Rosenshine, Ayala Livneh-Kol, Erez Gonen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Neutrophils
Mammary gland Interleukin-1beta Nitric Oxide Synthase Type II Inflammation [SDV.BC]Life Sciences [q-bio]/Cellular Biology macrophage Mastitis Biology medicine.disease_cause murine model 03 medical and health sciences Mice Mammary Glands Animal [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] medicine Escherichia coli Macrophage Animals Escherichia coli Infections 030304 developmental biology Mice Knockout 0303 health sciences General Veterinary 030306 microbiology [SDV.BA]Life Sciences [q-bio]/Animal biology Macrophages neutrophil Receptors Interleukin-1 Pathogenic bacteria [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Epithelium Toll-Like Receptor 4 [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics medicine.anatomical_structure [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Mammary Epithelium Gene Expression Regulation Immunology [SDV.IMM]Life Sciences [q-bio]/Immunology [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie Original Article Female medicine.symptom Pulmonary alveolus Signal Transduction |
| Zdroj: | Veterinary Research Veterinary Research, BioMed Central, 2010, 41 (4), ⟨10.1051/vetres/2010025⟩ |
| ISSN: | 1297-9716 0928-4249 |
| DOI: | 10.1051/vetres/2010025⟩ |
| Popis: | International audience; Mastitis, the inflammation of the mammary gland, is an important disease affecting dairy animals worldwide. The disease is caused by mammary pathogenic bacteria and Escherichia coli are frequently implicated. Virulence factors of mammary pathogenic E. coli are only partially known and intramammary challenge with LPS elicits neutrophil recruitment in experimental bovine and murine mastitis models. We have previously shown that neutrophil recruitment in LPS-induced murine mastitis is strictly dependent on mammary alveolar macrophages. However, the relative role of alveolar macrophages and blood neutrophils in E. coli mastitis is not well defined. To this end, we selectively depleted mammary alveolar macrophages or blood neutrophils before intramammary challenge with E. coli strain P4 (ECP4). Mice depleted of alveolar macrophages prior to intramammary challenge recruited neutrophils normally and restricted bacterial growth and interstitial invasion. Importantly however, upon depletion of alveolar macrophages, ECP4 invaded the mammary alveolar epithelial cells and formed intracellular bacterial communities. In contrast, neutrophil depletion prior to intramammary infection with ECP4 was associated with unrestricted bacterial growth, tissue damage, severe sepsis and mortality. This study suggests that neutrophils but not alveolar macrophages provide essential antimicrobial defense against mammary pathogenic E. coli. Furthermore, we show here similar invasion after depletion of alveolar macrophages as in our previous studies showing that LPS/TLR4 signaling on alveolar macrophages abrogates ECP4 invasion of the mammary epithelium. Interestingly, similar ECP4 invasion and formation of intracellular communities were also observed following intramammary infection of either iNOS gene-deficient or IL-1 receptor type 1 gene-deficient mice. |
| Databáze: | OpenAIRE |
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