Synthetic Promoters: Designing the cis Regulatory Modules for Controlled Gene Expression
| Autor: | Xiaowei Li, Li Haiyan, Jameel Aysha, Wang Fawei, Weican Liu, Muhammad Noman, Yonggang Zhou |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Computer science Gene Expression Bioengineering Computational biology Regulatory Sequences Nucleic Acid Gene engineering Applied Microbiology and Biotechnology Biochemistry 03 medical and health sciences Gene Expression Regulation Plant Gene expression Genes Synthetic Animals Promoter Regions Genetic Molecular Biology Gene Cis-regulatory module Regulation of gene expression Computational Biology Promoter Plants Human genetics 030104 developmental biology Genetic Engineering Biotechnology Cis-regulatory element |
| Zdroj: | Molecular Biotechnology. 60:608-620 |
| ISSN: | 1559-0305 1073-6085 |
| DOI: | 10.1007/s12033-018-0089-0 |
| Popis: | Designing the expression cassettes with desired properties remains the most important consideration of gene engineering technology. One of the challenges for predictive gene expression is the modeling of synthetic gene switches to regulate one or more target genes which would directly respond to specific chemical, environmental, and physiological stimuli. Assessment of natural promoter, high-throughput sequencing, and modern biotech inventory aided in deciphering the structure of cis elements and molding the native cis elements into desired synthetic promoter. Synthetic promoters which are molded by rearrangement of cis motifs can greatly benefit plant biotechnology applications. This review gives a glimpse of the manual in vivo gene regulation through synthetic promoters. It summarizes the integrative design strategy of synthetic promoters and enumerates five approaches for constructing synthetic promoters. Insights into the pattern of cis regulatory elements in the pursuit of desirable "gene switches" to date has also been reevaluated. Joint strategies of bioinformatics modeling and randomized biochemical synthesis are addressed in an effort to construct synthetic promoters for intricate gene regulation. |
| Databáze: | OpenAIRE |
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