CD109 represents a novel branch of the α2-macroglobulin/complement gene family
| Autor: | Melvin Chan, Parul Sharma, Paul T. Morrison, Robert W. Finberg, Keith R. Solomon |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
DNA
Complementary Sequence analysis Molecular Sequence Data Sequence alignment CHO Cells Biology GPI-Linked Proteins Evolution Molecular Exon Antigens CD Sequence Analysis Protein Cell Line Tumor Cricetinae Complementary DNA Genetics Animals Humans Gene family alpha-Macroglobulins Amino Acid Sequence Cloning Molecular Peptide sequence Phylogeny Genomic organization Sequence Homology Amino Acid Reverse Transcriptase Polymerase Chain Reaction Phosphatidylinositol Diacylglycerol-Lyase Intron Chromosome Mapping Complement System Proteins Exons Sequence Analysis DNA General Medicine Introns Neoplasm Proteins Genes Multigene Family Sequence Alignment |
| Zdroj: | Gene. 327:171-183 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2003.11.025 |
| Popis: | We report here the genomic organization and phylogenic relationships of CD109, a member of the the alpha2-macroglobulin/complement (AMCOM) gene family. CD109 is a GPI-linked glycoprotein expressed on endothelial cells, platelets, activated T-cells, and a wide variety of tumors. We cloned full-length CD109 cDNA from the mammalian U373 cell line by RT-PCR and performed analysis of its corresponding genomic sequence. The CD109 cDNA spans 128 kb of chromosome 6q with its 33 exons constituting approximately 3.3% of the total CD109 genomic sequence. Sequence analysis revealed that CD109 contains specific motifs in its N-terminus, that are highly conserved in all AMCOM members. CD109 also shares motifs with certain other AMCOM members including: (1) a thioester 'GCGEQ" motif, (2) a furin site of four positively charged amino acids, and (3) a double tyrosine near the C-terminus. Based on a phylogenic analysis of human CD109 with other human homologs as well as orthologs from other mammalian species, C. elegans (ZK337.1) and E. coli homologs, we propose CD109 represents a novel and independent branch of the alpha2-macroglobulin/complement gene family (AMCOM) and may be its oldest member. |
| Databáze: | OpenAIRE |
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