Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns
| Autor: | Andy M. Bailey, Claire Burns, Michael P. Challen, Meritxell Riquelme, Gary D. Foster, T.J. Elliott, M. Kirby, K.E. Gregory, M.-K. Cheung |
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| Rok vydání: | 2005 |
| Předmět: |
Agaricus
Green Fluorescent Proteins Restriction Mapping Biology Polymerase Chain Reaction Microbiology Green fluorescent protein Coprinus Genes Reporter Gene Expression Regulation Fungal Escherichia coli Genetics Cloning Molecular DNA Fungal Promoter Regions Genetic Gene DNA Primers Cloning Reporter gene Base Sequence Fungal genetics Promoter Plants Genetically Modified Molecular biology Introns Transformation (genetics) Biochemistry Agaricus bisporus |
| Zdroj: | Fungal Genetics and Biology. 42:191-199 |
| ISSN: | 1087-1845 |
| Popis: | We have developed a "Molecular Toolkit" comprising interchangeable promoters and marker genes to facilitate transformation of homobasidiomycete mushrooms. We describe the evaluation of a range of promoters in the homobasidiomycetes Agaricus bisporus and Coprinus cinereus using green fluorescent protein (GFP) as a reporter gene; the C. cinereus trp1 promoter and A. bisporus trp2 and gpdII promoters proving successful in driving expression in C. cinereus, with the gpdII promoter also functioning in A. bisporus. Our investigations demonstrate that a prerequisite for GFP expression in C. cinereus and A. bisporus is the presence of an intron. This is the first reported expression of GFP in either C. cinereus or A. bisporus. |
| Databáze: | OpenAIRE |
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