A disturbance of intestinal epithelial cell population and kinetics in APC1638T mice
| Autor: | Takao Senda, Takanori Onouchi, Shuji Matsuda, Tuya Wang, Nami Yamada |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Paneth Cells medicine.medical_specialty Adenomatous polyposis coli Enteroendocrine Cells Adenomatous Polyposis Coli Protein Population Gene Expression Cell Count Mice Transgenic Enteroendocrine cell Biology Pathology and Forensic Medicine Mice 03 medical and health sciences Protein Domains Intestinal mucosa Cell Movement Internal medicine Intestine Small medicine Animals Body Size Intestinal Mucosa education Molecular Biology Cell Proliferation Sequence Deletion Goblet cell education.field_of_study Base Sequence General Medicine Molecular biology Intestinal epithelium Small intestine 030104 developmental biology Endocrinology medicine.anatomical_structure Paneth cell biology.protein Female Goblet Cells |
| Zdroj: | Medical Molecular Morphology. 50:94-102 |
| ISSN: | 1860-1499 1860-1480 |
| DOI: | 10.1007/s00795-016-0152-5 |
| Popis: | The adenomatous polyposis coli (APC) is a multifunctional protein as well as a tumor suppressor. To determine the functions of the C-terminal domain of APC, we explored APC 1638T/1638T (APC1638T) mice that express a truncated APC lacking the C-terminal domain. The APC1638T mice were tumor free and exhibited growth retardation. In the present study, we compared small intestinal crypt-villus cells homeostasis in APC +/+ (WT) mice and APC1638T mice. The body weight of APC1638T mice was significantly smaller than that of WT mice at all ages. The length of small intestine of APC1638T mice was significantly shorter than that of WT mice. The crypt-villus axis was significantly elongated, and the number of intestinal epithelial cells also increased in APC1638T mice compared with those in WT mice. However, the number of intestinal epithelial cells per 100 µm of villi was not different between WT and APC1638T mice. Migration and proliferation of intestinal epithelial cells in APC1638T mice were faster than that in WT mice. The population of Goblet cells, Paneth cells, and enteroendocrine cells was significantly altered in APC1638T mice. These results indicate that C-terminal domain of APC has a role in the regulation of intestinal epithelium homeostasis. |
| Databáze: | OpenAIRE |
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