Observation of a Flowing Duct in the Abdominal Wall by Using Nanoparticles

Autor: Minseok Kim, HyunSuk Jang, Young-Jae Kim, Joohwan Yoon, HyunJi Gil, Jin-Kyu Lee, Kwang-Sup Soh, Sharon Jiyoon Jung
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Male
Pathology
0211 other engineering and technologies
H&E stain
lcsh:Medicine
02 engineering and technology
Abdominal cavity
Abdominal wall
Rats
Sprague-Dawley
0302 clinical medicine
Fluorescence Microscopy
Trichrome
Animal Cells
021105 building & construction
Abdomen
Medicine and Health Sciences
Nanotechnology
Mast Cells
Tolonium Chloride
Coloring Agents
Hematoxylin
lcsh:Science
Lymph node
Connective Tissue Cells
Staining
Microscopy
Multidisciplinary
Cell Staining
Light Microscopy
Abdominal Cavity
Anatomy
medicine.anatomical_structure
Connective Tissue
cardiovascular system
Eosine Yellowish-(YS)
Engineering and Technology
Peritoneum
Cellular Types
Rheology
Research Article
medicine.medical_specialty
Histology
Imaging Techniques
Abdominal Fat
Biology
Research and Analysis Methods
03 medical and health sciences
Fluorescence Imaging
medicine
Animals
Humans
Lymphatic Vessels
Staining and Labeling
Rhodamines
Abdominal Wall
lcsh:R
Biology and Life Sciences
Cell Biology
Rats
Biological Tissue
Specimen Preparation and Treatment
Cardiovascular Anatomy
Nanoparticles
Blood Vessels
lcsh:Q
Alcian Blue
Lymph Nodes
Duct (anatomy)
Acupuncture Points
030217 neurology & neurosurgery
Zdroj: PLoS ONE, Vol 11, Iss 3, p e0150423 (2016)
PLoS ONE
PLOS ONE(11): 3
ISSN: 1932-6203
Popis: The primo vascular system (PVS) is being established as a circulatory system that corresponds to acupuncture meridians. There have been two critical questions in making the PVS accepted as a novel liquid flowing system. The first one was directly to show the flow of liquid in PVS and the second one was to explain why it was not observed in the conventional histological study of animal tissues. Flow in the PVS in the abdominal cavity was previously verified by injecting Alcian blue into a primo node. However, the tracing of the dye to other subsystems of the PVS has not been done. In the current work we injected fluorescent nanoparticles (FNPs) into a primo node and traced them along a primo vessel which was inside a fat tissue in the abdominal wall. Linea alba is a white middle line in the abdominal skin of a mammal and a band of fat tissue is located in parallel to the linea alba in the parietal side of the abdominal wall of a rat. In this fat band a primo vessel runs parallel to the prominent blood vessels in the fat band and is located just inside the parietal peritoneum. About the second question on the reason why the PVS was not in conventional histological study the current work provided the answer. Histological analysis with hematoxyline and eosine, Masson’s trichrome, and Toluidine blue could not discriminate the primo vessel even when we knew the location of the PVS by the trace of the FNPs. This clearly explains why the PVS is hard to observe in conventional histology: it is not a matter of resolution but the contrast. The PVS has very similar structure to the connective tissues that surround the PVS. In the current work we propose a method to find the PVS: Observation of mast cell distribution with toluidine blue staining and the PN has a high density of mast cells, while the lymph node has low density.
Databáze: OpenAIRE
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