Mechanisms governing the responses to anthracnose pathogen in Juglans spp
| Autor: | M.E. Malvolti, G. van der Linden, A. Belisario, E. Turco, N. Anselmi, L. Luongo, Irene Olimpieri, Alberto Santini, G. Scarascia Mugnozza, Maria Gras, Paola Pollegioni |
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| Rok vydání: | 2012 |
| Předmět: |
Host specific resistance
Walnut binding DNA Plant Genotype black-walnut Juglans Bioengineering Biology Plant disease resistance Applied Microbiology and Biotechnology Statistics Nonparametric Botany NBS-profiling Colletotrichum Cluster Analysis Pathogen Disease Resistance Plant Diseases General Medicine Nucleic acid amplification technique biology.organism_classification Plant Leaves Plant Breeding Horticulture Phenotype Gnomonia leptostyla population-genetics quantitative trait loci Host-Pathogen Interactions Avoidance by late flushing identification Microsatellite powdery mildew disease resistance genes regia l bud burst Nucleic Acid Amplification Techniques medicago-truncatula Powdery mildew Microsatellite Repeats Biotechnology |
| Zdroj: | Journal of Biotechnology 159 (2012) 4 Journal of biotechnology 159 (2012): 251–264. doi:10.1016/j.jbiotec.2011.08.020 info:cnr-pdr/source/autori:Pollegioni P., Van der Linden G., Belisario A., Gras M., Anselmi N., Olimpieri I., Luongo L., Santini A., Turco E., Scarascia Mugnozza G., Malvolti M.E./titolo:Mechanisms governing the responses to anthracnose pathogen in Juglans spp./doi:10.1016%2Fj.jbiotec.2011.08.020/rivista:Journal of biotechnology/anno:2012/pagina_da:251/pagina_a:264/intervallo_pagine:251–264/volume:159 Journal of Biotechnology, 159(4), 251-264 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2011.08.020 |
| Popis: | Juglans nigra and Juglans regia are two highly economically important species for wood and fruit production that are susceptible to anthracnose caused by Gnomonia leptostyla. The identification of genotypes resistant to anthracnose could represent a valid alternative to agronomic and chemical management. In this study, we analyzed 72 walnut genotypes that showed a variety of resistance phenotypes in response to natural infection. According to the disease severity rating and microsatellite fingerprinting analysis, these genotypes were divided into three main groups: (40) J. nigra resistant, (1) J. nigra susceptible, and (31) J. regia susceptible. Data on leaf emergence rates and analysis of in vivo pathogenicity indicated that the incidence of anthracnose disease in the field might be partially conditioned by two key factors: the age and/or availability of susceptible leaves during the primary infection of fungus (avoidance by late flushing) and partial host resistance. NBS profiling approach, based on PCR amplification with an adapter primer for an adapter matching a restriction enzyme site and a degenerate primer targeting the conserved motifs present in the NBS domain of NBS-LRR genes, was applied. The results revealed the presence of a candidate marker that correlated to a reduction in anthracnose incidence in 72 walnut genotypes. |
| Databáze: | OpenAIRE |
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