Short Tandem Repeat analysis after Whole Genome Amplification of single B-lymphoblastoid cells
| Autor: | Jana Weymaere, Filip Van Nieuwerburgh, Dieter Deforce, Ann-Sophie Vander Plaetsen, Lieselot Deleye |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Str markers lcsh:Medicine Computational biology Biology Genome Article 03 medical and health sciences Cell Line Tumor Humans lcsh:Science Whole Genome Amplification B-Lymphocytes Multidisciplinary Genome Human Lymphoblast lcsh:R Biology and Life Sciences Str profiling STR Profile 030104 developmental biology Cell culture Microsatellite lcsh:Q Reagent Kits Diagnostic Single-Cell Analysis Nucleic Acid Amplification Techniques Microsatellite Repeats |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-6 (2018) SCIENTIFIC REPORTS |
| ISSN: | 2045-2322 |
| Popis: | To allow multiple genetic analyses on a single cell, whole genome amplification (WGA) is required. Unfortunately, studies comparing different WGA methods for downstream human identification Short Tandem Repeat (STR) analysis remain absent. Therefore, the aim of this work was to assess the performance of four commercially available WGA kits for downstream human identification STR profiling on a B-lymphoblastoid cell line. The performance was assessed using an input of one or three micromanipulated cells. REPLI-g showed a very low dropout rate, as it was the only WGA method in this study that could provide a complete STR profile in some of its samples. Although Ampli1, DOPlify and PicoPLEX did not detect all selected STR markers, they seem suitable for genetic identification in single-cell applications. |
| Databáze: | OpenAIRE |
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