B-50/GAP43 localization on membranes of putative transport vesicles in the cell body, neurites and growth cones of cultured hippocampal neurons
| Autor: | A.J. Verkleij, A.B. Oestreicher, W.H. Gispen, C.G. Dotti, M. Van Lookeren Campagne |
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| Rok vydání: | 1992 |
| Předmět: |
Neurite
Golgi Apparatus Nerve Tissue Proteins Hippocampal formation Hippocampus symbols.namesake GAP-43 Protein Microtubule Organelle Neurites Animals Growth cone Microscopy Immunoelectron Cells Cultured Neurons Organelles Membrane Glycoproteins biology General Neuroscience Vesicle Biological Transport Intracellular Membranes Golgi apparatus Primary and secondary antibodies Immunohistochemistry Axons Cell biology Rats biology.protein symbols |
| Zdroj: | Neuroscience letters. 137(1) |
| ISSN: | 0304-3940 |
| Popis: | We conducted an electron microscopic immunocytochemical study to locate B-50/GAP43 in various cellular elements of hippocampal neurons grown in dissociated cell culture. B-50 was detected with pre- and post-embedding immunoincubation, using affinity-purified B-50 antibodies and secondary antibodies coated on gold probes. For the first time ultrastructural evidence is presented for the location of B-50 on the membranes of electron-lucent transport vesicles with a diameter of 99.4 +/- 2.9 nm, present in the trans region of the Golgi apparatus, in neurites and in growth cones of cultured hippocampal neurons. |
| Databáze: | OpenAIRE |
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