Effects of long-termin vitrocultivation on the virulence of cloned lines ofLeishmania majorpromastigotes
| Autor: | Manuel Segovia, E. Mellado, M. L. Chance, J. M. Artero |
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| Rok vydání: | 1992 |
| Předmět: |
Time Factors
030231 tropical medicine Population Clone (cell biology) Leishmaniasis Cutaneous Virulence Mice 03 medical and health sciences 0302 clinical medicine 030225 pediatrics Animals Leishmania major education Cloning Infectivity Mice Inbred BALB C education.field_of_study biology Inoculation Macrophages biology.organism_classification Virology Clone Cells Infectious Diseases Leishmania tropica Protozoa Female Parasitology |
| Zdroj: | Annals of Tropical Medicine & Parasitology. 86:347-354 |
| ISSN: | 1364-8594 0003-4983 |
| DOI: | 10.1080/00034983.1992.11812677 |
| Popis: | The virulences of several clones from a single Leishmania major strain were studied in BALB/c mice. Clones showed the same pattern of infectivity and virulence two months after cloning as the parental population. After prolonged in vitro culture, however, it was apparent that two types of virulent clones existed: although the level of virulence remained stable in some clones, in others, such as C-11, it progressively decreased, as in the parental population. The progressive loss in virulence in a continuously cultured mixed population was probably due to selection, as the initial mixture of a stable virulent clone and a stable avirulent clone eventually yielded a totally avirulent promastigote population. After cultivation for 12 months, neither clone C-11 nor the parental population produced lesions in inoculated mice but virulent parasites were recovered from the inguinal nodes of the mice, possibly as a result of selection in vivo for virulent parasites. |
| Databáze: | OpenAIRE |
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