A Pencil-Lead Immunosensor for the Rapid Electrochemical Measurement of Anti-Diphtheria Toxin Antibodies
| Autor: | Maiara O. Salles, Thiago R.L.C. Paixão, Eric T. Costa, David William Provance, Vanessa N. Ataide, Paloma Napoleão-Pêgo, Salvatore Giovanni De-Simone, Larissa Rodrigues Gomes, Wilson A. Ameku |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Clinical Biochemistry
Peptide Enzyme-Linked Immunosorbent Assay Biosensing Techniques biosensor Immunoglobulin G Epitope Article EPITOPOS Epitopes Diphtheria Toxin diphtheria Detection limit Diphtheria toxin chemistry.chemical_classification Immunoassay electrochemistry square wave voltammetry B-cell epitope point-of-care Chromatography biology Chemistry General Medicine Reagent biology.protein Antibody Biosensor TP248.13-248.65 Biotechnology |
| Zdroj: | Biosensors; Volume 11; Issue 12; Pages: 489 Biosensors Biosensors, Vol 11, Iss 489, p 489 (2021) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 2079-6374 |
| DOI: | 10.3390/bios11120489 |
| Popis: | Diphtheria is a vaccine-preventable disease, yet immunization can wane over time to non-protective levels. We have developed a low-cost, miniaturized electroanalytical biosensor to quantify anti-diphtheria toxin (DTx) immunoglobulin G (anti-DTx IgG) antibody to minimize the risk for localized outbreaks. Two epitopes specific to DTx and recognized by antibodies generated post-vaccination were selected to create a bi-epitope peptide, biEP, by synthesizing the epitopes in tandem. The biEP peptide was conjugated to the surface of a pencil-lead electrode (PLE) integrated into a portable electrode holder. Captured anti-DTx IgG was measured by square wave voltammetry from the generation of hydroquinone (HQ) from the resulting immunocomplex. The performance of the biEP reagent presented high selectivity and specificity for DTx. Under the optimized working conditions, a logarithmic calibration curve showed good linearity over the concentration range of 10−5–10−1 IU mL−1 and achieved a limit of detection of 5 × 10−6 IU mL−1. The final device proved suitable for interrogating the immunity level against DTx in actual serum samples. Results showed good agreement with those obtained from a commercial enzyme-linked immunosorbent assay. In addition, the flexibility for conjugating other capture molecules to PLEs suggests that this technology could be easily adapted to the diagnoses of other pathogens. |
| Databáze: | OpenAIRE |
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