In Vitro Assessment of Axonal Growth Using Dorsal Root Ganglia Explants in a Novel Three-Dimensional Collagen Matrix
| Autor: | Frank Schügner, Ingo Heschel, Ahmet Bozkurt, Christina Beckmann, F. Lassner, Sven Moellers, Leon Olde Damink, Michael Woeltje, Norbert Pallua, Paul Fuchs, Gary A. Brook, Joachim Weis, Julian Tank, Bernd Sellhaus |
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| Rok vydání: | 2007 |
| Předmět: |
Scaffold
Cell Culture Techniques Cell Enlargement Matrix (biology) Tissue engineering Ganglia Spinal medicine Animals Cells Cultured Tissue Engineering biology Guided Tissue Regeneration Chemistry Regeneration (biology) General Engineering Anatomy Axons In vitro Nerve Regeneration Rats medicine.anatomical_structure nervous system Rats Inbred Lew Cell culture Peripheral nervous system biology.protein Biophysics Collagen Neurotrophin |
| Zdroj: | Tissue Engineering. 13:2971-2979 |
| ISSN: | 1557-8690 1076-3279 |
| Popis: | The goal of this study was the development of a bioartificial nerve guide to induce axonal regeneration in the peripheral nervous system (PNS). In this in vitro study, the ability of a novel, 3-dimensional (3D), highly oriented, cross-linked porcine collagen scaffold to promote directed axonal growth has been studied. Collagen nerve guides with longitudinal guidance channels were manufactured using a series of chemical and mechanical treatments with a patented unidirectional freezing process, followed by freeze-drying (pore sizes 20-50 microm). Hemisected rat dorsal root ganglia (DRG) were positioned such that neural and non-neural elements could migrate into the collagen scaffold. After 21 days, S100-positive Schwann cells (SCs) migrated into the scaffold and aligned within the guidance channels in a columnar fashion, resembling "Bands of Büngner." Neurofilament-positive axons (mean length +/- SD 756 microm +/- 318 microm, maximum 1496 microm) from DRG neurons entered the scaffold where the growth within the guidance channels was closely associated with the oriented SCs. This study confirmed the importance of SCs in the regeneration process (neurotrophic theory). The alignment of SCs within the guidance channels supported directional axonal growth (contact guidance theory). The microstructural properties of the scaffold (open, porous, longitudinal pore channels) and the in vitro data after DRG loading (axonal regeneration along migrated and columnar-aligned SCs resembling "Band of Büngner") suggest that this novel oriented 3D collagen scaffold serves as a basis for future experimental regeneration studies in the PNS. |
| Databáze: | OpenAIRE |
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