Determination of 3′-azido-3′-deoxythymidine, 2′,3′-dideoxycytidine, 3′-fluoro-3′-deoxythymidine and 2′,3′-dideoxyinosine in biological samples by high-performance liquid chromatography
| Autor: | Hans Christoph Michaelis, G.F. Kahl, Nicole Frijus-Plessen, H. Foth |
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| Rok vydání: | 1990 |
| Předmět: |
Antiviral Agents
030226 pharmacology & pharmacy High-performance liquid chromatography 2'3' dideoxyinosine 03 medical and health sciences 0302 clinical medicine 2'3' dideoxycytidine Animals Humans 3 azido 3 deoxythymidine Chromatography High Pressure Liquid 030304 developmental biology Detection limit 0303 health sciences Chromatography Zalcitabine Chemistry Extraction (chemistry) General Chemistry 3'-fluoro-3'-deoxythymidine Dideoxynucleosides Rats Didanosine Liver Zidovudine Nucleoside |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 534:101-107 |
| ISSN: | 0378-4347 |
| Popis: | A simple and fast high-performance liquid chromatographic assay for the determination of 3′-azido-3′-deoxythymidine (AZT), 2′,3′-dideoxycytidine (ddC), 3′-fluoro-3′-deoxythymidine (FT) and 2′,3′-dideoxyinosine (ddI) in complex biological matrices is described. The method allows rapid nucleoside determination using a phenyl column within extra- as well as intracellular media without further sample pretreatment and extraction procedures. The lower limit of detection is ca. 0.05 μg/ml for each nucleoside, and the separation can easily be optimized for AZT, ddC, FT and ddI by variation of the methanolic part of the mobile phase and the detector wavelengths. |
| Databáze: | OpenAIRE |
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