Heparin blocks transfer of extracellular vesicles between donor and recipient cells
| Autor: | Xandra O. Breakefield, Henk A. van Veen, Nadia A. Atai, Casey A. Maguire, Cornelis J.F. Van Noorden, Leonora Balaj, Peter A. Jarzyna, Johan Skog |
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| Přispěvatelé: | AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, CCA -Cancer Center Amsterdam, Cell Biology and Histology |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
media_common.quotation_subject Biology Real-Time Polymerase Chain Reaction Article law.invention Flow cytometry Confocal microscopy law Tumor Cells Cultured medicine Extracellular Humans RNA Messenger Transport Vesicles Internalization media_common Messenger RNA medicine.diagnostic_test Heparin Reverse Transcriptase Polymerase Chain Reaction Vesicle Anticoagulants Flow Cytometry Microvesicles Cell biology ErbB Receptors Neurology Oncology Biochemistry Neurology (clinical) Extracellular Space Glioblastoma medicine.drug |
| Zdroj: | Journal of neuro-oncology, 115(3), 343-351. Kluwer Academic Publishers |
| ISSN: | 0167-594X |
| DOI: | 10.1007/s11060-013-1235-y |
| Popis: | Extracellular vesicles (EVs) have been implicated in tumorigenesis. Biomolecules which can block EV binding and uptake into recipient cells may be of therapeutic value as well as enhance understanding of EV biology. Here, we show that heparin interacts with uptake of tumor-derived as well as non-tumor-derived EVs into recipient cells. Incubation of glioma cell-derived EVs with heparin resulted in micron-sized structures observed by transmission electron microscopy, with EVs clearly visible within these structures. Inclusion of heparin greatly diminished transfer of labeled EVs from donor to recipient tumor cells. We also show a direct interaction between heparin and EVs using confocal microscopy. We found that the block in EV uptake was at the level of cell binding and not internalization. Finally, incubation of glioma-derived EVs containing EGFRvIII mRNA with heparin reduced transfer of this message to recipient cells. The effect of heparin on EVs uptake may provide a unique tool to study EV function. It may also foster research of heparin or its derivatives as a therapeutic for disease in which EVs play a role. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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