A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells
| Autor: | Jong Seung Kim, Sankarprasad Bhuniya, Inseob Shim, Miae Won, Kondapa Naidu Bobba, Zhigang Yang, Nithya Velusamy, Junle Qu |
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| Rok vydání: | 2017 |
| Předmět: |
Boron Compounds
Cell Survival Tyrosinase 010402 general chemistry 01 natural sciences Catalysis Fluorescence chemistry.chemical_compound Mice Two-photon excitation microscopy Cell Line Tumor Materials Chemistry Animals Humans Tyrosinase activity Fluorescent Dyes Photons Molecular Structure 010405 organic chemistry Monophenol Monooxygenase Optical Imaging Metals and Alloys Rational design Reproducibility of Results General Chemistry Hydrogen Peroxide 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Up-Regulation Microscopy Fluorescence Multiphoton Biochemistry chemistry Cell culture Ceramics and Composites BODIPY Intracellular HeLa Cells |
| Zdroj: | Chemical communications (Cambridge, England). 53(81) |
| ISSN: | 1364-548X |
| Popis: | Herein, we report rational design, synthesis, and application of a two-photon fluorescent probe (Tyro-1) for tracking intracellular tyrosinase activity. The chemoselective detection of tyrosinase is precluded from interference of other competitive omnipresent oxidizing entities in cellular milieu. The probe showed 12.5-fold fluorescence enhancement at λem = 450 nm in the presence of tyrosinase. The nontoxic probe Tyro-1 provides information about H2O2-mediated upregulation of tyrosinase through cellular imaging. Its two-photon imaging ability makes it a noninvasive tool for validating the expression of tyrosinase in the live cells. |
| Databáze: | OpenAIRE |
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