Three New Phthalide Glycosides from the Rhizomes of Cnidium officinale and Their Recovery Effect on Damaged Otic Hair Cells in Zebrafish
| Autor: | Seon Min Oh, Trong Nguyen Nguyen, Youn Hee Nam, Nam-In Baek, Ji Heon Shim, Tong Ho Kang, Min-Ho Lee, Na Woo Kim, Hyoung-Geun Kim, Dae Young Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Circular dichroism
animal structures Cnidium officinale circular dichroism ligusticoside otic hair cells phthalide stereostructure zebrafish Metabolite Pharmaceutical Science Organic chemistry Analytical Chemistry Phthalide chemistry.chemical_compound Column chromatography QD241-441 Drug Discovery medicine Physical and Theoretical Chemistry Zebrafish chemistry.chemical_classification Chromatography biology Chemistry Glycoside Neomycin biology.organism_classification Rhizome Chemistry (miscellaneous) embryonic structures Molecular Medicine sense organs medicine.drug |
| Zdroj: | Molecules; Volume 26; Issue 22; Pages: 7034 Molecules, Vol 26, Iss 7034, p 7034 (2021) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules26227034 |
| Popis: | The extract from Cnidium officinale rhizomes was shown in a prior experiment to markedly recover otic hair cells in zebrafish damaged by neomycin. The current study was brought about to identify the principal metabolite. Column chromatography using octadecyl SiO2 and SiO2 was performed to isolate the major metabolites from the active fraction. The chemical structures were resolved on the basis of spectroscopic data, including NMR, IR, MS, and circular dichroism (CD) data. The isolated phthalide glycosides were assessed for their recovery effect on damaged otic hair cells in neomycin-treated zebrafish. Three new phthalide glycosides were isolated, and their chemical structures, including stereochemical characteristics, were determined. Two glycosides (0.1 μM) showed a recovery effect (p < 0.01) on otic hair cells in zebrafish affected by neomycin ototoxicity. Repeated column chromatography led to the isolation of three new phthalide glycosides, named ligusticosides C (1), D (2), and E (3). Ligusticoside C and ligusticoside E recovered damaged otic hair cells in zebrafish. |
| Databáze: | OpenAIRE |
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