Nasal or intramuscular immunization of mice with influenza subunit antigen and the B subunit of Escherichia coli heat-labile toxin induces IgA- or IgG-mediated protective mucosal immunity
| Autor: | Marijke Holtrop, E Agsteribbe, WR Verweij, De Haan Lolke, Ruud Brands, A. M. Palache, Guus J. M. van Scharrenburg, Jan Wilschut |
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| Rok vydání: | 2001 |
| Předmět: |
ADJUVANT ACTIVITY
Orthomyxoviridae Bacterial Toxins VACCINE VIRUS-INFECTION medicine.disease_cause Antibodies Viral Injections Intramuscular TRACE AMOUNT Immunoglobulin G Microbiology Enterotoxins Mice Antigen Adjuvants Immunologic medicine INTRANASAL IMMUNIZATION Animals Lung Administration Intranasal Mice Inbred BALB C General Veterinary General Immunology and Microbiology biology Immunogenicity Escherichia coli Proteins Cholera toxin Public Health Environmental and Occupational Health CHOLERA-TOXIN biology.organism_classification ADP-RIBOSYLATION ACTIVITY Rats ENTEROTOXIN Nasal Mucosa Protein Subunits Infectious Diseases ANTIBODY Immunization Influenza Vaccines Humoral immunity Immunology Immunoglobulin A Secretory biology.protein IMMUNOGLOBULIN-G Molecular Medicine Female Antibody |
| Zdroj: | Vaccine, 19(20-22), 2898-2907. ELSEVIER SCI LTD |
| ISSN: | 0264-410X |
| Popis: | Local mucosal IgA antibodies play a central role in protection of the respiratory tract against influenza virus infection. Therefore, new-generation influenza vaccines should aim at stimulating not only systemic, but also local antibody responses. Previously, we demonstrated that the recombinant B subunit of the Escherichia coli heat-labile toxin (LTB) is a potent adjuvant towards nasally administered influenza subunit antigen. Here, we investigated the protection conferred by LTB-supplemented influenza subunit antigen given intranasally (i.n.) or intramuscularly (i.m.) to mice. Both i.n. and i.m. immunization with subunit antigen and LTB completely protected the animals against viral infection. Protection upon i.n. immunization was associated with the induction of antigen-specific serum IgG and mucosal IgA, whereas protection upon i.m. immunization correlated with strong serum and mucosal IgG, but not IgA responses. We conclude that LTB-supplemented influenza subunit antigen, given either i.n. or i.m, induces protective antibody-mediated mucosal immunity and thus represents a promising novel flu vaccine candidate. (C) 2001 Elsevier Science Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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