Exposing dairy bulls to high temperature-humidity index during spermatogenesis compromises subsequent embryo development in vitro
| Autor: | Luana de Cássia Bicudo, Daniel de Souza Ramos Angrimani, Núria Llamas Luceño, Katarzyna Joanna Szymańska, Luc Peelman, Erik Mullaart, Kristel Demeyere, Evelyne Meyer, Ann Van Soom, Mario Van Poucke, Marleen L.W.J. Broekhuijse |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Hot Temperature Cell Survival Embryonic Development Semen Fertilization in Vitro Biology Semen collection Embryo Culture Techniques Andrology 03 medical and health sciences Semen quality 0302 clinical medicine Food Animals medicine Animals Veterinary Sciences Blastocyst Spermatogenesis Small Animals Heat index 030219 obstetrics & reproductive medicine urogenital system Equine 0402 animal and dairy science Humidity 04 agricultural and veterinary sciences Spermatozoa 040201 dairy & animal science Sperm medicine.anatomical_structure Gene Expression Regulation Sperm Motility Cattle Animal Science and Zoology Embryo quality |
| Zdroj: | Theriogenology THERIOGENOLOGY |
| ISSN: | 0093-691X 1879-3231 |
| DOI: | 10.1016/j.theriogenology.2019.08.034 |
| Popis: | The possible impact of natural heat stress on animal fertility is currently a major concern for breeding companies. Here, we aimed to address this concern by determining the effects of natural heat stress on the fertility of Holstein bulls located in the Netherlands. Semen samples were collected from six bulls at two locations in March 2016 (low temperature-humidity index (THI) group; maximum THI of 51.8 and 55 at their respective locations) or August (high THI group; maximum THI of 77.9 and 80.5 during meiotic and spermiogenic stages of spermatogenesis, 42 to 14 days prior to semen collection). The effect of heat stress on semen quality was assessed by sperm morphology, motility, reactive oxygen species production, lipid peroxidation, viability, and DNA fragmentation. Moreover, we evaluated the development of embryos generated in vitro by low and high THI semen, and determined inner cell mass/trophectoderm ratio, apoptotic cell ratio, and embryonic gene expression in day-8 blastocysts. An increase in cell death (propidium iodide-positive cells; P = 0.039) was observed in the high THI group (31.5%) compared to the low THI group (27.6%). Moreover, a decrease (P |
| Databáze: | OpenAIRE |
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