Macrophage Polarization in IL-10 Treatment of Particle-Induced Inflammation and Osteolysis
| Autor: | Bin Ning, Jianhao Jiang, Weiming Gong, Shang-You Yang, Paul H. Wooley, Tanghong Jia |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Osteolysis Blotting Western Macrophage polarization Inflammation Real-Time Polymerase Chain Reaction Pathology and Forensic Medicine Nitric oxide Mice 03 medical and health sciences chemistry.chemical_compound Postoperative Complications Image Processing Computer-Assisted medicine Animals Macrophage Arthroplasty Replacement Mice Inbred BALB C Macrophages X-Ray Microtomography Macrophage Activation medicine.disease Immunohistochemistry Interleukin-10 Disease Models Animal Interleukin 10 030104 developmental biology chemistry Immunology Cancer research Female Polyethylenes Signal transduction medicine.symptom CD163 Signal Transduction |
| Zdroj: | The American Journal of Pathology. 186:57-66 |
| ISSN: | 0002-9440 |
| Popis: | This study investigated the therapeutic influence and potential mechanism of IL-10 in ameliorating orthopedic debris particle-induced inflammation and osteolysis. A murine air pouch with bone implantation and polyethylene particles was also used to evaluate the therapeutic effects of IL-10. The data suggested that the particle challenges significantly promoted macrophage activation and osteoclastogenesis, with dramatically increased macrophage infiltration into the pouch membranes and elevated tartrate-resistant acid phosphatase-positive cell deposition. Immunohistochemical stains revealed a significantly higher ratio of induced nitric oxide synthase-expressing cells in the particle-challenged group; treatment with IL-10 resulted in marked switching to CD163(+) cells. Also, IL-10 effectively reduced tartrate-resistant acid phosphatase-positive stained cells in the pouch membranes, and minimized the bone mineral density loss compared with untreated samples. Real-time PCR and Western blot examination indicated that IL-10 treatment significantly diminished the particle-induced IL-1β expression but promoted expression of CD163, transforming growth factor-β1, and CCR2. Furthermore, IL-10 significantly inhibited the ultra-high-molecular-weight polyethylene particle-elevated phospho-STAT1 and phospho-NF-κB p65 productions, and promoted phospho-STAT3 expression. Overall, the data indicate the pivotal effects of IL-10 on macrophage polarization. The effects of IL-10 in ameliorating local inflammation and osteolysis may be associated with macrophage polarization through the up-regulation of the Janus activating kinase/STAT3 signaling pathway, and the down-regulation of NF-κB and Janus activating kinase/STAT1 expression. |
| Databáze: | OpenAIRE |
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