Early postnatal development of the immune system in piglets: The redistribution of T lymphocyte subsets
| Autor: | Lenka Leva, H. Stepanova, P. Samankova, M. Faldyna, J. Sinkora |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.diagnostic_test
biology Lymphoid Tissue Swine CD3 Immunology Double negative Receptors Antigen T-Cell gamma-delta Spleen Flow Cytometry Immunohistochemistry Flow cytometry Andrology medicine.anatomical_structure Immune system Animals Newborn T-Lymphocyte Subsets Immune System medicine biology.protein Animals Lymph CD8 |
| Zdroj: | Cellular Immunology. 249:73-79 |
| ISSN: | 0008-8749 |
| Popis: | In this study, we have characterised postnatal changes in T lymphocyte subsets, especially gammadelta T lymphocytes, in blood, spleen and lymph nodes. Detection was carried out using two-colour flow cytometry and three-colour immunohistochemistry. During ontogeny, there was a significant increase in the total percentage of gammadelta T cells in the spleen and blood. In the lymph nodes, there were no age-dependent changes in the total percentage of gammadelta T cells, but the percentage of the gammadeltaTCR+CD8+ subpopulation significantly increased. The tissue distribution of gammadeltaTCR+CD8+ and gammadeltaTCR+CD8- cells in the lymph nodes is random and not collocated with a particular area of the organ. Furthermore, postnatal development was characterised by an increasing frequency of CD8+CD3+CD4-gammadeltaTCR-, which was compensated by a decreasing proportion of CD4+ lymphocytes. Double positive CD4+CD8+ lymphocytes were rare during the first month of life and a significant age-dependent increase of these cells was found in all the compartments monitored. |
| Databáze: | OpenAIRE |
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