Biodiversity of exopolysaccharides produced by Streptococcus thermophilus strains is reflected in their production and their molecular and functional characteristics
| Autor: | Luc De Vuyst, Marc Vancanneyt, Jean Swings, Fernanda Mozzi, Frederik Vaningelgem, Tom Adriany, Medana Zamfir |
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| Rok vydání: | 2004 |
| Předmět: |
Streptococcus thermophilus
EXOPOLYSACCHARIDES Rhamnose Bacterial growth Polysaccharide Applied Microbiology and Biotechnology Ciencias de la Tierra y relacionadas con el Medio Ambiente Gel permeation chromatography Ciencias Biológicas chemistry.chemical_compound STREPTOCOCCUS THERMOPHILUS Structure-Activity Relationship Biología Celular Microbiología Yeast extract Animals Food science chemistry.chemical_classification Ecology biology Polysaccharides Bacterial Streptococcus Biodiversity biology.organism_classification Culture Media Milk chemistry Biochemistry Galactose Ciencias Medioambientales Fermentation Food Microbiology Dimerization CIENCIAS NATURALES Y EXACTAS Food Science Biotechnology |
| Zdroj: | Applied and environmental microbiology. 70(2) |
| ISSN: | 0099-2240 |
| Popis: | Twenty-six lactic acid bacterium strains isolated from European dairy products were identified as Streptococcus thermophilus and characterized by bacterial growth and exopolysaccharide (EPS)-producing capacity in milk and enriched milk medium. In addition, the acidification rates of the different strains were compared with their milk clotting behaviors. The majority of the strains grew better when yeast extract and peptone were added to the milk medium, although the presence of interfering glucomannans was shown, making this medium unsuitable for EPS screening. EPS production was found to be strain dependent, with the majority of the strains producing between 20 and 100 mg of polymer dry mass per liter of fermented milk medium. Furthermore, no straightforward relationship between the apparent viscosity and EPS production could be detected in fermented milk medium. An analysis of the molecular masses of the isolated EPS by gel permeation chromatography revealed a large variety, ranging from 10 to >2,000 kDa. A distinction could be made between high-molecular-mass EPS (>1,000 kDa) and low-molecular-mass EPS ( |
| Databáze: | OpenAIRE |
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