Expression of retinoblastoma gene product and p21 (WAF1/Cip 1) protein in gliomas: correlations with proliferation markers, p53 expression and survival
| Autor: | Konstantinos Kouzelis, Athanassios Papanikolaou, Penelope Korkolopoulou, Euphemia Thomas-Tsagli, Panayota Christodoulou |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Cyclin-Dependent Kinase Inhibitor p21 Male Adolescent Oligodendroglioma Nerve Tissue Proteins Astrocytoma Retinoblastoma Protein Disease-Free Survival Pathology and Forensic Medicine Gene product Cellular and Molecular Neuroscience Cyclins Proliferating Cell Nuclear Antigen Glioma Gene expression medicine Humans Life Tables Genes Retinoblastoma neoplasms Survival analysis Aged Regulation of gene expression biology Brain Neoplasms Retinoblastoma Middle Aged Genes p53 Prognosis medicine.disease Survival Analysis Proliferating cell nuclear antigen Gene Expression Regulation Neoplastic Ki-67 Antigen biology.protein Cancer research Female Neurology (clinical) Tumor Suppressor Protein p53 Biomarkers Cell Division |
| Zdroj: | Acta Neuropathologica. 95:617-624 |
| ISSN: | 1432-0533 0001-6322 |
| DOI: | 10.1007/s004010050848 |
| Popis: | Using immunohistochemistry we evaluated the expression of two negative regulators of the cell cycle, the retinoblastoma gene product (pRb) and the WAF1/Cip1 gene product (p21), in consecutive paraffin sections from 54 gliomas (49 astrocytomas and 5 oligodendrogliomas) and related it to clinicopathological parameters, proliferative fraction, p53 expression and survival. Survival analysis was restricted to the group of diffuse astrocytomas (48 patients). pRb expression did not correlate with histological type, grade or p53 expression, while a moderately strong correlation existed between pRb expression and the percentages of proliferating cell nuclear antigen (PCNA) and MIB-1-positive cells. In 30% of cases we observed diminished pRb expression (i.e., a low pRb/Ki-67 ratio), irrespective of grade or histological type. p21 protein was elevated in 50% of cases, especially within the higher grades. The percentage of p21-positive cells was not related to histological type or grade but correlated loosely with PCNA and pRb expression. A p53-negative/p21-negative phenotype was characteristic of oligodendrogliomas and low-grade astrocytomas, whereas the p53-positive/p21-positive, p53-positive/p21-negative and p53-negative/p21-positive phenotypes were almost equally distributed among high-grade tumors. In survival analysis (either univariate or multivariate) diminished pRb expression was not a statistically significant prognostic indicator. In contrast, p21 expression emerged as an important indicator of shortened disease-free survival, in both univariate and multivariate analyses. Moreover, the double-positive p53/p21 phenotype tended to be associated with a shorter overall survival. Our results suggest that Rb gene deregulation does not significantly affect prognosis but p21 expression may play an important role in disease-free survival of astrocytoma patients. |
| Databáze: | OpenAIRE |
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