Does a Porcine Hepatocyte Hybrid Artificial Liver Prolong the Survival Time of Anhepatic Rabbits?
| Autor: | MANABU TAKAMASHI, HIROKAZU MATSUE, MICHIAKI MATSUSHITA, KAZUHIRO SATO, MAKOTO NISHIKAWA, MASAHIKO KOIKE, HIROMITSU NOTO, YASUAKI NAKAJIMA, JUNICHI UCHINO, TAKASHI KOMAI, ETSURO HASHIMURA |
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| Rok vydání: | 1992 |
| Předmět: |
Swine
medicine.medical_treatment Transplantation Heterologous Biomedical Engineering Biophysics Bioengineering In Vitro Techniques Biology Liver transplantation Biomaterials Andrology Perfusion Culture medicine Animals Cells Cultured Lagomorpha Gluconeogenesis General Medicine biology.organism_classification In vitro Liver Transplantation Artificial organ medicine.anatomical_structure Liver Evaluation Studies as Topic Hepatocyte Immunology Artificial Organs Rabbits Perfusion Ex vivo |
| Zdroj: | ASAIO Journal. 38:M468-M472 |
| ISSN: | 1058-2916 |
| Popis: | To examine cultured porcine hepatocytes as a bioreactor of a hybrid artificial liver (HAL) in rabbits, a small version of a multiplated HAL was manufactured. In vitro and ex vivo studies were performed to evaluate the small HAL. The HAL consisted of 50 glass plates (10 x 5 x 0.04 cm each) on which porcine hepatocytes were cultured in a monolayer at confluent cell density. After 2 days of standard cultivation, the glass plates with hepatocytes were placed in the module and were used for studies. The module contains about 5 grams of hepatocytes (1/10 of 2 kg.bw rabbit liver). After undergoing perfusion culture for 5 days, the HAL showed satisfactory hepatic function. Glucose and urea were synthesized at the rate of 4.06 +/- 0.7 mg/module/hr and 0.62 +/- 0.09 mg/module/hr, respectively, and loaded ammonia was metabolized at the rate of 1.29 +/- 0.26 mg/module/hr. Ex vivo extracorporeal plasma perfusion studies revealed that the module with cultured porcine hepatocytes, which were heterogeneous to rabbits, showed a tendency to prolong the survival time of anhepatic rabbits. These results indicate that the HAL system, using multiplated porcine hepatocyte monolayers, is a promising artificial liver support system for clinical cases. |
| Databáze: | OpenAIRE |
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