An electrical bio-chip to transfer and detect electromagnetic stimulation on the cells based on vertically aligned carbon nanotubes
| Autor: | Saeed Rafizadeh-Tafti, Mohammad Abdolahad, Mohsen Janmaleki, Mohammad Hossein Haqiqatkhah, Somayeh Zanganeh, Mehrdad Saviz, Reza Faraji Dana |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Cell Survival Bioengineering Nanotechnology 02 engineering and technology Carbon nanotube 010402 general chemistry 01 natural sciences Electric charge Electromagnetic radiation Fluorescence Cell Line law.invention Biomaterials Electricity law Cell Line Tumor Microscopy Humans Biochip Electrodes Nanotubes Carbon 021001 nanoscience & nanotechnology 0104 chemical sciences Membrane Mechanics of Materials Dielectric Spectroscopy Electrode Biophysics 0210 nano-technology Electromagnetic Phenomena Biosensor |
| Zdroj: | Materials Science and Engineering: C. 70:681-688 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2016.09.050 |
| Popis: | A highly sensitive impedimetric bio-chip based on vertically aligned multiwall carbon nanotubes (VAMWCNTs), was applied in direct interaction with lung cancer cells. Our tool provided both inducing and monitoring the bioelectrical changes in the cells initiated by electromagnetic (EM) wave stimulation. EM wave of 940 MHz frequency with different intensities was used. Here, wave ablation might accumulate electrical charge on the tips of nanotubes penetrated into cell's membrane. The charge might induce ionic exchanges into the cell and cause alterations in electrical states of the membrane. Transmembrane electrostatic/dynamic states would be strongly affected due to such exchanges. Our novel modality was that, the cells' vitality changes caused by charge inductions were electrically detected with the same nanotubes in the architecture of electrodes for impedance measurement. The responses of the sensor were confirmed by electron and florescent microscopy images as well as biological assays. In summation, our method provided an effective biochip for enhancing and detecting external EM stimulation on the cells useful for future diagnostic and therapeutic applications, such as wave-guided drug-resistance breakage. |
| Databáze: | OpenAIRE |
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