Impaired Akt phosphorylation in B-cells of patients with common variable immunodeficiency
| Autor: | Nima Rezaei, Zohre Sharifi, Asghar Aghamohammadi, Gholamreza Azizi, Reza Yazdani, Abbas Rezaei, Hassan Abolhassani, Mazdak Ganjalikhani-Hakemi, Mohammad Esmaeili, Shahram Vaeli |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Adolescent Immunology B-Lymphocyte Subsets Biology Flow cytometry 03 medical and health sciences Phosphatidylinositol 3-Kinases Young Adult medicine Immunology and Allergy Humans Phosphorylation Child Protein kinase B PI3K/AKT/mTOR pathway B cell B-Lymphocytes medicine.diagnostic_test Common variable immunodeficiency medicine.disease Marginal zone Flow Cytometry 030104 developmental biology medicine.anatomical_structure Common Variable Immunodeficiency Apoptosis Primary immunodeficiency Female Immunologic Memory Proto-Oncogene Proteins c-akt |
| Zdroj: | Clinical immunology (Orlando, Fla.). 175 |
| ISSN: | 1521-7035 |
| Popis: | Common variable immunodeficiency (CVID) is a heterogeneous group of primary immunodeficiency characterized by recurrent infections. We evaluated whether defective PI3K/Akt/FoxO pathway could influence B-cell fate. Determination of B-cell subsets in CVD patients and healthy donors (HDs) were performed using flow cytometry. We evaluated mRNA and protein expression of PI3K, Akt and FoxO using real-time PCR and flow cytometry, respectively. Moreover, phosphorylated Akt (pAkt) expression in B-cells has been measured by flowcytometry. We identified a significant reduction in the percentage of marginal zone like B-cells, memory B-cells (total, switched and unswitched) and plasmablasts in patients, as these decreased B-cell subsets had a significant negative correlation with increased apoptosis in patients. Surprisingly, we identified decreased pAkt expression in B-cells of patients than HDs. We described for the first time impaired pAkt expression in B-cells of CVID patients that had a significant correlation with antibody response to the vaccine and worse clinical complications. |
| Databáze: | OpenAIRE |
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