6K2-induced vesicles can move cell to cell during turnip mosaic virus infection
| Autor: | Huanquan Zheng, Jean-François Laliberté, Jun Jiang, Romain Grangeon, Juan Wan, Maxime Agbeci |
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| Přispěvatelé: | Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP), Department of Biology [Montréal], McGill University = Université McGill [Montréal, Canada], This study was supported by the Natural Sciences and Engineering Research Council of Canada and from Le Fonds de recherche du Québec – Nature et technologies to Huanquan Zheng and Jean-François Laliberté. |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Microbiology (medical) viruses [SDV]Life Sciences [q-bio] Cell Potyvirus Plant RNA virus lcsh:QR1-502 membrane association Plasmodesma Biology 01 natural sciences Microbiology intercellular movement lcsh:Microbiology Green fluorescent protein Cell wall 03 medical and health sciences chemistry.chemical_compound RNA polymerase medicine Turnip mosaic virus replication complex Original Research Article 030304 developmental biology 0303 health sciences Vesicle biology.organism_classification Virology 3. Good health medicine.anatomical_structure chemistry Intracellular 010606 plant biology & botany |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Frontiers Media, 2013, 4, pp.351. ⟨10.3389/fmicb.2013.00351⟩ Frontiers in Microbiology, Vol 4 (2013) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2013.00351⟩ |
| Popis: | International audience; To successfully infect plants, viruses replicate in an initially infected cell and then move to neighboring cells through plasmodesmata (PDs). However, the nature of the viral entity that crosses over the cell barrier into non-infected ones is not clear. The membrane-associated 6K2 protein of turnip mosaic virus (TuMV) induces the formation of vesicles involved in the replication and intracellular movement of viral RNA. This study shows that 6K2-induced vesicles trafficked toward the plasma membrane and were associated with plasmodesmata (PD). We demonstrated also that 6K2 moved cell-to-cell into adjoining cells when plants were infected with TuMV. 6K2 was then fused to photo-activable GFP (6K2:PAGFP) to visualize how 6K2 moved intercellularly during TuMV infection. After activation, 6K2:PAGFP-tagged vesicles moved to the cell periphery and across the cell wall into adjacent cells. These vesicles were shown to contain the viral RNA-dependent RNA polymerase and viral RNA. Symplasmic movement of TuMV may thus be achieved in the form of a membrane-associated viral RNA complex induced by 6K2. |
| Databáze: | OpenAIRE |
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