Characterisation of verotoxin-producing Escherichia coli strains isolated from human patients in Hungary over a 7-year period
| Autor: | N. Nógrády, M. Herpay, Ferenc Rozgonyi, T. Mag, Istvan Toth |
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| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
Serotype Genotype Virulence Factors Microbial Sensitivity Tests Biology medicine.disease_cause beta-Lactamases Microbiology fluids and secretions medicine Humans Serotyping Escherichia coli Escherichia coli Infections Intimin Hungary Shiga-Toxigenic Escherichia coli Toxin Escherichia coli Proteins General Medicine Antimicrobial biology.organism_classification Virology Enterobacteriaceae Anti-Bacterial Agents Bacterial adhesin Infectious Diseases VTEC |
| Zdroj: | European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 29(2) |
| ISSN: | 1435-4373 |
| Popis: | The purpose of this study was to characterise verotoxigenic Escherichia coli (VTEC) strains isolated in Hungary from 2000 to 2006. Altogether, 33 human VTEC strains were investigated to define the O:H antigens, verotoxin 1, 2 (vtx1 and 2), intimin (eae), enteroaggregative heat-stable toxin (ast1), autoagglutinating adhesin (saa) and enterohaemolysin (ehlyA) genes and sensitivity to 11 antimicrobial agents. The strains belonged to 14 different O:H serotypes, among which O157:NM (non-motile) was the most prevalent (45%, 15/33). Patients infected with O157 more often presented bloody diarrhoea or haemorrhagic colitis (63%, 12/19) than those infected with non-O157 (46%, 6/14). Haemolytic uraemic syndrome evolved in two patients infected with O26:H11. The vtx1vtx2c toxin gene combination was found in 58% (11/19) and vtx2c alone in 31% (6/19) of the O157 strains. All of the O157 strains possessed gamma1, while two O26 strains had the beta1 intimin gene. Twenty strains (75%, 25/33) carried the ehlyA gene and five non-O157 strains had ast1. The majority of the strains (76%) were resistant to at least one antimicrobial agent, but none of them showed the extended-spectrum beta-lactamase (ESBL) phenotype. |
| Databáze: | OpenAIRE |
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