The role of fibroblasts in the modulation of dental pulp inflammation
| Autor: | Shan Ling Hung, Ya Yun Lee, Shue Fen Yang, Yi-Ching Ho, Chia Lun Tsai |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Medicine (General)
Cellular differentiation Inflammation Monocytes Proinflammatory cytokine 03 medical and health sciences 0302 clinical medicine R5-920 Osteoprotegerin Dental pulp fibroblasts Medicine Tumor necrosis factor-alpha Humans Dental Pulp biology business.industry Interleukin General Medicine Fibroblasts Molecular biology RANKL 030220 oncology & carcinogenesis biology.protein Leukocytes Mononuclear Pulp (tooth) 030211 gastroenterology & hepatology Tumor necrosis factor alpha medicine.symptom business |
| Zdroj: | Journal of the Formosan Medical Association, Vol 121, Iss 1, Pp 342-349 (2022) |
| ISSN: | 0929-6646 |
| Popis: | Background/purpose Dental pulp fibroblasts can protect dental pulp from microbial invasion. However, little is known about the interaction between pulp fibroblasts and the immune cells. In this study, the production of proinflammatory cytokines related to inflammatory cell recruitment was evaluated in tumor necrosis factor (TNF)-α-stimulated human dental pulp fibroblasts (HDPFs). The role of TNF-α-stimulated HDPFs in the cell fusion under inflammatory process was determined with the cell co-culture with peripheral blood mononuclear cells (PBMCs). Methods HDPFs were stimulated with various concentrations of TNF-α, and the secretion of interleukin (IL)-6, IL-8 and monocyte chemoattractant protein (MCP)-1 was analyzed by the enzyme-linked immunosorbent assay. The mRNA expression levels of intercellular adhesion molecule-1 (ICAM-1), macrophage colony-stimulating factor (M-CSF), receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegerin (OPG) were determined by real-time quantitative polymerase chain reaction. TNF-α-treated HDPFs were co-cultured with PBMCs for 21 days, and characteristics of cell differentiation were assessed. Results TNF-α induced IL-6, IL-8 and MCP-1 production in HDPFs. Moreover, mRNA expression levels of ICAM-1, M-CSF and OPG were significantly increased in TNF-α-treated HDPFs. Co-culture of TNF-α-treated HDPFs and PBMCs stimulated formation of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells, and the F-actin rings were observed in these multinucleated cells. Conclusion Our results indicate that under the stimulation of TNF-α, HDPFs may amplify inflammatory response by cytokines production, which in turn can modulate the differentiation of immune cells. |
| Databáze: | OpenAIRE |
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