Expression of a human cytomegalovirus receptor correlates with infectibility of cells
| Autor: | T Compton, Neil R. Cooper, D M Nowlin |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Human cytomegalovirus
viruses Immunology Cytomegalovirus Biology Microbiology DNA-binding protein Virus Cell Line Viral Envelope Proteins Viral entry Virology medicine Animals Humans Receptor medicine.disease Cell biology Molecular Weight Membrane glycoproteins Membrane protein Cell culture Insect Science Cytomegalovirus Infections biology.protein Receptors Virus Research Article |
| Zdroj: | Journal of Virology. 65:3114-3121 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.65.6.3114-3121.1991 |
| Popis: | Previous studies have demonstrated that human cytomegalovirus (HCMV) specifically binds to a fibroblast membrane glycoprotein(s) with a molecular mass from 30 to 34 kDa. In this study, the distribution of the putative receptor proteins was analyzed in a variety of cell types, including cell types representative of those that are infected in vivo. Using a sensitive microbinding assay (to score virus attachment) and an indirect detection method (to score HCMV-binding proteins), we found that the 34- and 32-kDa HCMV binding proteins are ubiquitous molecules, broadly distributed among diverse cell types. In addition, the level of virus attachment was found to correlate with the abundance of the 34- and 32-kDa cellular proteins, while the ability of the virus to penetrate cells and initiate infection did not. The results support the hypothesis that the 34- and 32-kDa cellular proteins represent the HCMV (attachment) receptor. The data also support the notion that additional cellular components are required for virus entry and fusion. |
| Databáze: | OpenAIRE |
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