Effects of topical FK506 on endotoxin-induced uveitis (EIU) in the Lewis rat
| Autor: | Scott M. Whitcup, Naofumi Hikita, Manabu Mochizuki, Chi-Chao Chan, Robert B. Nussenblatt |
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| Rok vydání: | 1995 |
| Předmět: |
Drug
Salmonella typhimurium medicine.drug_class media_common.quotation_subject medicine.medical_treatment Administration Topical Antibiotics Pharmacology Tacrolimus Aqueous Humor Immunoenzyme Techniques Cellular and Molecular Neuroscience Anterior Eye Segment polycyclic compounds Medicine Animals Endotoxin induced uveitis Eye Proteins media_common Dose-Response Relationship Drug business.industry Therapeutic effect medicine.disease Uveitis Anterior Sensory Systems Rats Endotoxins Ophthalmology Dose–response relationship Disease Models Animal Rats Inbred Lew Immunology Prednisolone Female Ophthalmic Solutions business Cell Adhesion Molecules Uveitis medicine.drug Topical steroid |
| Zdroj: | Current eye research. 14(3) |
| ISSN: | 0271-3683 |
| Popis: | FK506 is a macrolide antibiotic and a potent immunosuppressant. To investigate the effect of topical FK506 on acute ocular inflammation, we evaluated its action on the development of endotoxin-induced uveitis (EIU). At two different concentrations of 0.05% and 0.3%, topical FK506 was applied to Lewis rats with EIU. In aqueous, the mean number of inflammatory cells per microliter +/- SEM was 2,389 +/- 1,277, 1,571 +/- 1,562, 898 +/- 882, and 69 +/- 152 for rats treated with vehicle alone, 0.05%, 0.3% FK506, and 1% prednisolone acetate. The median of histological grades was 2, 1.5, 0.8, and 0.5 for animals treated with these 4 different regimens respectively. Analysis of aqueous protein showed a small reduction in FK506-treated animals. Mean blood levels of FK506 were low in rats treated with topical FK506 (0.05%, 0.84 ng/ml; 0.3%, 2.0 ng/ml) suggesting that its therapeutic effect was not secondary to the systemic absorption of the drug. Although FK506 is not as effective as prednisolone, 0.3% FK506 produced significant decreases in the mean aqueous inflammatory cell number and histological inflammatory score as compared to control vehicle alone. We conclude that topical FK506 can suppress EIU in a dose-dependent fashion and may be an alternative medication for patients with anterior uveitis and contra-indication to topical steroid. |
| Databáze: | OpenAIRE |
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