Coupled RNA processing and transcription of intergenic primary microRNAs
| Jazyk: | English |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93fd71f3158f81d16bd7ff731fe7fd45 https://ora.ox.ac.uk/objects/uuid:54d0a1db-eed8-4143-8dc4-f8af93df404f |
| Rights: | OPEN |
| Přírůstkové číslo: | edsair.doi.dedup.....93fd71f3158f81d16bd7ff731fe7fd45 |
| Autor: | Marcella Marchioni, Francesca Pagano, Mariangela Morlando, Irene Bozzoni, Erika Girardi, Davide Cacchiarelli, Nick J. Proudfoot, Monica Ballarino |
| Přispěvatelé: | Ballarino, Monica, Pagano, Francesca, Girardi, Erika, Morlando, Mariangela, Cacchiarelli, Davide, Marchioni, Marcella, Proudfoot, Nicholas J., Bozzoni, Irene |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Ribonuclease III
RNA Processing DGCR8 Messenger Post-Transcriptional RNA polymerase II RNA-Binding Protein Primary transcript HeLa Cell Cell Line Microprocessor complex Promoter Regions Genetic Small Nuclear Cell Line Tumor RNA Small Nuclear Humans RNA Messenger RNA Processing Post-Transcriptional Promoter Regions Genetic RNA polymerase II holoenzyme Molecular Biology Drosha Tumor biology General transcription factor Protein Proteins RNA-Binding Proteins MicroRNA Cell Biology Articles Non-coding RNA Molecular biology Exoribonuclease MicroRNAs Exoribonucleases HeLa Cells RNA Polymerase II biology.protein RNA Human |
| Popis: | The first step in microRNA (miRNA) biogenesis occurs in the nucleus and is mediated by the Microprocessor complex containing the RNase III-like enzyme Drosha and its cofactor DGCR8. Here we show that the 5'-->3' exonuclease Xrn2 associates with independently transcribed miRNAs and, in combination with Drosha processing, attenuates transcription in downstream regions. We suggest that, after Drosha cleavage, a torpedo-like mechanism acts on nascent long precursor miRNAs, whereby Xrn2 exonuclease degrades the RNA polymerase II-associated transcripts inducing its release from the template. While involved in primary transcript termination, this attenuation effect does not restrict clustered miRNA expression, which, in the majority of cases, is separated by short spacers. We also show that transcripts originating from a miRNA promoter are retained on the chromatin template and are more efficiently processed than those produced from mRNA or snRNA Pol II-dependent promoters. These data imply that coupling between transcription and processing promotes efficient expression of independently transcribed miRNAs. |
| Databáze: | OpenAIRE |
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