Distribution maps of D-dopachrome tautomerase in the mouse brain
| Autor: | Eduard I. Dedkov, Lin Leng, Joerg R. Leheste, German Torres, Jared S. Honigman, Richard Bucala, Kristin M. DiGregorio |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Blotting Western Fluorescent Antibody Technique Antibodies Gene product Mice Animals Peptide sequence Cellular localization chemistry.chemical_classification Brain Mapping Innate immune system biology General Neuroscience Brain Immunohistochemistry Blot Intramolecular Oxidoreductases Mice Inbred C57BL Enzyme chemistry Biochemistry Polyclonal antibodies Data Interpretation Statistical biology.protein Macrophage migration inhibitory factor Electrophoresis Polyacrylamide Gel Female |
| Zdroj: | Neuroscience. 226 |
| ISSN: | 1873-7544 |
| Popis: | D-Dopachrome tautomerase is an enzyme related by amino acid sequence and catalytic activity to macrophage migration inhibitory factor. Both of these small molecules are pro-inflammatory cytokines mediating broad innate immune responses. Although it is well established that the gene product of D-dopachrome tautomerase is widely expressed in liver and kidney cells, no study has mapped the distribution pattern of this tautomeric enzyme in the mammalian nervous system. Here, we address this void by characterizing the cellular localization of D-dopachrome tautomerase in the adult mouse brain. Two well-characterized polyclonal antibodies were used for Western blotting and immunohistochemical localization of the endogenous tautomeric enzyme. Our results show that D-dopachrome tautomerase is present throughout the brain parenchyma with a large fraction of heterogeneous interneurons harboring a stable and robust expression of the enzyme. These data point to a potential involvement of D-dopachrome tautomerase activity in the mature mouse brain, and suggest some functional and evolutionary relationship between innate immunity and tautomerization of D-dopachrome in mammalian species. |
| Databáze: | OpenAIRE |
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