Quantitation of the next-generation imipridone ONC206 in human plasma by a simple and sensitive UPLC-MS/MS assay for clinical pharmacokinetic application

Autor:	Jennifer C. Goodell, Sara M. Zimmerman, Cody J. Peer, Varun Prabhu, Tyler Yin, William J. Richardson, Arya Azinfar, John A. Dunn, Mark Mullin, Brett J. Theeler, Mark Gilbert, William D. Figg
Rok vydání:	2021
Předmět:	Tandem Mass Spectrometry Clinical Biochemistry Drug Discovery Pharmaceutical Science Humans Reproducibility of Results Antineoplastic Agents Spectroscopy Chromatography High Pressure Liquid Article Analytical Chemistry Chromatography Liquid
Zdroj:	J Pharm Biomed Anal
ISSN:	1873-264X
Popis:	ONC206 is an imipridone derivative that is being developed clinically as a single agent given orally in a first-in-human trial (NCT04541082). This ongoing clinical trial requires pharmacokinetic analysis of ONC206 to fully characterize its pharmacologic profile. There is currently no published bioanalytical method for ONC206 quantitation. To understand the clinical pharmacokinetics of ONC206, a sensitive yet simple uHPLC-MS/MS method for quantitation of ONC206 in human plasma was developed. Protein-precipitation allowed rapid and sensitive bioanalytical measurement of ONC206 in human plasma. A Phenomenex Kinetex C18 (50 ×2.1 mm, 1.3 µm, 100 Å) analytical column achieved symmetrical and sharp chromatography peaks of ONC206 and the internal standard, [
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93fa4f4582aaacc7e2e325f03f88ef0e https://pubmed.ncbi.nlm.nih.gov/35219065 Zobrazit plný text záznamu Full Text from ScienceDirect