Cytotoxic and apoptotic effects of 1,2-diborolanes with strong donor substitutes on human cancer cells
| Autor: | Yüksel Şahin, Kubilay Metin, Muhittin Aygün, Erkan Fırıncı, Özlem Sultan Aslantürk, Hüseyin Özgener, Tülay Aşkin Çelik, Resul Sevinçek |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Boron Compounds
Cell Survival medicine.medical_treatment Cell Antineoplastic Agents Apoptosis Biochemistry Structure-Activity Relationship Drug Discovery Tumor Cells Cultured medicine Humans Cytotoxic T cell Molecular Biology Cell Proliferation Membrane Potential Mitochondrial Membrane potential Chemotherapy Dose-Response Relationship Drug Molecular Structure Chemistry Organic Chemistry Cancer medicine.disease medicine.anatomical_structure Cancer cell Cancer research Drug Screening Assays Antitumor Liver cancer |
| Popis: | In recent years, boron compounds have become more common as chemotherapy agents against certain types of cancers. Along with the development of boron-based therapeutic agents have come investigations into the various cancers and biochemical and molecular mechanisms affected by boron compounds and the relationships between boron compounds and chemical protection against cancer. In this preliminary study, the effects of new 1,2-N-substituted-1,2-diborolane derivatives on types of breast and liver cancers were examined for the first time. Four were found to significantly affect the cell viabilities and mitochondrial membrane potential changes in MCF-7, HepG2 and Hep3B cancer cells. Each was prepared in n-hexane at various concentrations (5, 10, 25, 50, 75 and 100 µg/mL). Human peripheral blood lymphocytes were used as control cells. Compounds 1, 2, 3a, and 3b 1,2-diborolane derivatives selectively killed cancer cells, but compound 1 was cytotoxic in a concentration-dependent manner on HepG2 and Hep3B and only at concentrations of at least 75 µg/mL on MCF-7 cells. Compound 3a exhibited cytotoxic effect on lymphocytes at 75 and 100 µgmL−1 concentrations, but compounds 1, 2 and 3b, 3c and 3d have not possessed significant cytotoxic effect on lymphocytes. Compounds 3c and 3d have not possessed significant cytotoxic effects. Mitochondrial membrane potential assay results supported these findings. Our results reveal that 1,2-diborolane derivates have high cytotoxic and apoptotic activities on human hepatocarcinoma cells and are therefore potential candidates in the development of new drugs against liver cancer. |
| Databáze: | OpenAIRE |
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