Enzyme Immunoassay for Tenuazonic Acid in Apple and Tomato Products
| Autor: | Hadri Latif, Ewald Usleber, Madeleine Gross, Yvonne Ackermann, V. Curtui |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Malus
Tenuazonic Acid Food Contamination Antibodies Beverages Immunoenzyme Techniques chemistry.chemical_compound Solanum lycopersicum medicine Tenuazonic acid Animals Mycotoxin Detection limit Chromatography biology medicine.diagnostic_test Succinic anhydride food and beverages General Chemistry Alternaria biology.organism_classification chemistry Fruit Immunoassay biology.protein Rabbits General Agricultural and Biological Sciences Keyhole limpet hemocyanin |
| Zdroj: | Journal of Agricultural and Food Chemistry. 59:12317-12322 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf203540y |
| Popis: | The Alternaria mycotoxin tenuazonic acid was derivatized with succinic anhydride and conjugated to keyhole limpet hemocyanin (KLH) and to horseradish peroxidase (HRP), respectively. The KLH conjugate was used to produce polyclonal antibodies in rabbits. A competitive direct enzyme immunoassay (EIA) for tenuazonic acid was established, which was moderately sensitive for tenuazonic acid [50% inhibition concentration (IC(50)): 320 ± 130 ng/mL] but strongly reacted with tenuazonic acid acetate (IC(50): 23.3 ± 7.5 ng/mL). Therefore, an optimized EIA protocol was established, which employed acetylation of standard and sample extract solutions. The mean standard curve detection limit (IC(30)) for tenuazonic acid acetate was 5.4 ± 2.0 ng/mL, enabling detection limits for tenuazonic acid in apple and tomato products of 25-50 ng/g (150 ng/g in tomato paste). Recoveries in a concentration range of 50-2000 ng/g were 60-130% in apple juice and tomato juice and 40-150% in other tomato products. Tenuazonic acid was detected in apple juice and tomato products from German retail shops at levels of 50-200 ng/g. In conclusion, this novel EIA for tenuazonic acid could be useful within a screening program for Alternaria mycotoxins in food. |
| Databáze: | OpenAIRE |
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