Modulation of protein adsorption, vascular cell selectivity and platelet adhesion by mussel-inspired surface functionalization
| Autor: | Sherry Li Xu, Nan Huang, Meng Yang, Yang Leng, Xiong Lu, Cathy W. C. Bi, Zhilu Yang, Jingcheng Zhang, Pingbo Huang, Yonghui Ding |
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| Rok vydání: | 2014 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Stress fiber Materials science Biocompatibility health care facilities manpower and services education Cell Biomedical Engineering General Chemistry General Medicine engineering.material Cell selectivity Focal adhesion medicine.anatomical_structure Coating health services administration Biophysics medicine engineering Surface modification Organic chemistry General Materials Science Protein adsorption |
| Zdroj: | J. Mater. Chem. B. 2:3819-3829 |
| ISSN: | 2050-7518 2050-750X |
| Popis: | A mussel-inspired surface functionalization of the polydopamine (PDA) coating has been demonstrated to be a promising strategy to ensure the biocompatibility of various biomaterials. To explore the multifunctionality of the PDA coating for vascular stents and elucidate the mechanisms by which the PDA coating modulates vascular cell behavior, this study examined the protein adsorption, the responses of endothelial cells (ECs) and smooth muscle cells (SMCs), and platelet adhesion to various PDA-coated surfaces synthesized at varied initial dopamine concentrations. Our results indicate that various PDA coatings present distinct and varied functionalities. The quinone group on the PDA coating induces a substantially higher amount of protein adsorption, which subsequently plays a key role in promoting EC attachment and proliferation by regulating their focal adhesion and stress fiber formation. Meanwhile, the reactive phenolic hydroxyl group on the PDA coating potently inhibits SMC proliferation. In addition, the quinone-regulated fibrinogen adsorption to the PDA coating may increase platelet adhesion. Notably, the PDA coating synthesized at an initial dopamine concentration of 1.0 g L−1 shows the most favorable vascular cell selectivity. These findings shed light on the relationships between surface characteristics, protein adsorption, vascular cell behavior, and platelet adhesion of the PDA coating, which may guide better design of PDA application in vascular stents. |
| Databáze: | OpenAIRE |
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