The molecular basis of different approaches for the study of cancer stem cells and the advantages and disadvantages of a three-dimensional culture
| Autor: | José M. Alvarez-Suarez, Eduardo Garcia Villena, Johura Ansary, Kilian Tutusaus Pifarre, Tamara Y. Forbes-Hernandez, Lucia Regolo, Denise Quinzi, Danila Cianciosi, Francesca Giampieri, Maurizio Battino, Santos Gracia Villar |
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| Rok vydání: | 2021 |
| Předmět: |
cancer stem cells
enrichment Population CD34 Pharmaceutical Science Computational biology Cell Separation Review Biology Analytical Chemistry Flow cytometry Immunophenotyping surface molecular markers 03 medical and health sciences QD241-441 0302 clinical medicine Cancer stem cell Cell Line Tumor Spheroids Cellular three-dimensional culture Drug Discovery medicine Tumor Cells Cultured Animals Humans Physical and Theoretical Chemistry education 3207.03 Carcinogénesis 030304 developmental biology Cell specific 0303 health sciences education.field_of_study 2407.01 Cultivo Celular medicine.diagnostic_test functional assays Organic Chemistry Myeloid leukemia methodology Flow Cytometry Rare tumor 3201.01 Oncología Chemistry (miscellaneous) 030220 oncology & carcinogenesis Neoplastic Stem Cells Molecular Medicine identification Identification (biology) isolation Biomarkers |
| Zdroj: | Investigo. Repositorio Institucional de la Universidade de Vigo Universidade de Vigo (UVigo) Molecules Molecules, Vol 26, Iss 2615, p 2615 (2021) |
| Popis: | Cancer stem cells (CSCs) are a rare tumor subpopulation with high differentiation, proliferative and tumorigenic potential compared to the remaining tumor population. CSCs were first discovered by Bonnet and Dick in 1997 in acute myeloid leukemia. The identification and isolation of these cells in this pioneering study were carried out through the flow cytometry, exploiting the presence of specific cell surface molecular markers (CD34+/CD38−). In the following years, different strategies and projects have been developed for the study of CSCs, which are basically divided into surface markers assays and functional assays; some of these techniques also allow working with a cellular model that better mimics the tumor architecture. The purpose of this mini review is to summarize and briefly describe all the current methods used for the identification, isolation and enrichment of CSCs, describing, where possible, the molecular basis, the advantages and disadvantages of each technique with a particular focus on those that offer a three-dimensional culture. |
| Databáze: | OpenAIRE |
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