Maximizing the Diagnostic Yield from Bone Marrow Aspirate Material Using the Cell Block Technique on Clot Sections
| Autor: | Mary Lowery-Nordberg, Sudha Pillarisetti, Menchu Ong, Diana M Veillon, James D. Cotelingam |
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| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.diagnostic_test business.industry Biopsy Biochemistry (medical) Clinical Biochemistry Hematopoietic Tissue Myeloid leukemia Nucleated Red Blood Cell Bone Marrow Cells In situ hybridization Staining medicine.anatomical_structure Molecular Diagnostic Techniques Bone Marrow Myelodysplastic Syndromes Humans Medicine Immunohistochemistry Bone marrow business |
| Zdroj: | Laboratory Medicine. 46:e24-e27 |
| ISSN: | 1943-7730 0007-5027 |
| Popis: | Background: Paraffin section of bone-marrow aspirate (clot section) is one of several components of bone marrow biopsy. Improper acquisition of aspirate material results in lack of diagnostic tissue and a waste of resources. Objective: To detail a novel cell block method of aspirated marrow as a way to ensure maximum yield. This is of particular value when the material gathered via core biopsy is inadequate. Method: We used the cell block method to evaluate paraffin-embedded sections of hematopoietic tissue from bone marrow aspirate. Results: The range of diagnoses possible from an adequate clot section parallels those from a comparable core biopsy. Examples of an adequate clot section include lesions assessed by routine hematoxylin-eosin (H&E) staining, special and immunohistochemistry (IHC) stains, and molecular diagnostic studies such as fluorescent in situ hybridization (FISH) and polymerase chain reaction (PCR). Conclusion: A bone marrow clot section comprised of sinusoidal blood is inadequate for morphological interpretation and is a waste of resources. Hence, we recommend the cell block technique for procurement; this method ensures maximum capture of material needed to establish a diagnosis. * EDTA : ethylenediaminetetraacetic acid IHC : immunohistochemistry H&E : hematoxylin-eosin ALIP : abnormally located immature precursors AML : acute myeloid leukemia RSB : ring sideroblasts NRBC : nucleated red blood cells FISH : fluorescent in situ hybridization PCR : polymerase chain reaction EGFR : epidermal growth factor receptor |
| Databáze: | OpenAIRE |
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