Extreme Descemet's membrane rupture with hydrops in keratoconus: Clinical and histological manifestations
| Autor: | Charles N J McGhee, I-Ping Loh, Jennifer C Fan Gaskin, Trevor Sherwin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Pathology
medicine.medical_specialty Keratoconus Histology genetic structures 03 medical and health sciences Type IV collagen 0302 clinical medicine lcsh:Ophthalmology Laminin Cornea Case report medicine Specific staining Descemet's membrane biology business.industry Hydrops medicine.disease eye diseases Corneae Ophthalmology medicine.anatomical_structure lcsh:RE1-994 030221 ophthalmology & optometry biology.protein Descemet's membrane rupture sense organs business Descemet's tear 030217 neurology & neurosurgery |
| Zdroj: | American Journal of Ophthalmology Case Reports American Journal of Ophthalmology Case Reports, Vol 10, Iss, Pp 271-275 (2018) |
| ISSN: | 2451-9936 |
| Popis: | Purpose: To study the clinical and histological manifestations of an extreme Descemet's membrane rupture as a result of keratoconus. Observations: Using Periodic acid-Schiff assay to study a keratoconic cornea with an extreme rupture showed that the ruptured Descemet's membrane had retracted and folded into scrolls and ridges. The dimensions of the rupture were estimated to be 3.7mm2, and the central cornea was extremely thinned with a thickness of only 260μm. Stromal scarring and loosely packed lamellae were present anterior to the scrolls and ridges. Antibodies targetting the major components of Descemet's membrane, Laminin and type IV collagen, displayed intense labelling adjacent to the scrolls where the stroma was denuded and differential expression patterns lined the ridges. Environmental scanning electron microscopy showed possible collagen deposition at the site of rupture. Conclusions and importance: The specific staining patterns of laminin and type IV collagen suggest these components have an important role in re-endothelisation of the cornea. This is the first known report of spatial resolution of the topography of the Descemet's membrane rupture established by environmental scanning electron microscopic image montage. Keywords: Keratoconus, Descemet's membrane, Descemet's tear, Hydrops, Corneae, Histology |
| Databáze: | OpenAIRE |
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