Development and evaluation of a multiplex polymerase chain reaction assay to identify Salmonella serogroups and serotypes
| Autor: | Miryan Margot Sánchez-Jiménez, Nélida Muñoz, Jaime A. Moreno, Nora Cardona-Castro, Lelia Lavalett |
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| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
Serotype Salmonella Reproducibility of Results General Medicine Biology bacterial infections and mycoses medicine.disease_cause biology.organism_classification Polymerase Chain Reaction Virology Enterobacteriaceae Microbiology Infectious Diseases Bacterial Proteins Salmonella enterica Multiplex polymerase chain reaction medicine Typing Serotyping DNA Primers |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 65:327-330 |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/j.diagmicrobio.2009.07.003 |
| Popis: | To improve limitations of Salmonella serotyping, 2 multiplex polymerase chain reaction (M-PCR) were developed using a strategy that identifies first the genes encoding serogroups (rfbJ, wzx). According to the serogroup determined, a second M-PCR identifies serotype (fliC, fljB, wcdB, and sdf-I sequence). Standardization and evaluation of both M-PCRs were carried out. |
| Databáze: | OpenAIRE |
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