Acutely transforming avian leukosis virus subgroup J strain 966: defective genome encodes a 72-kilodalton Gag-Myc fusion protein
| Autor: | Ken Howes, L. N. Payne, J. C. McKay, P. M. Chesters, K. Venugopal |
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| Rok vydání: | 2001 |
| Předmět: |
Transforming virus
viruses Immunology Genes myc Gene Products gag Genome Viral Biology Microbiology Defective virus Virus Transformation and Oncogenesis Cell Line Transduction (genetics) Transformation Genetic Proviruses Virology Animals Deoxyribonucleases Type II Site-Specific Gene Oncogene Avian Leukosis Virus Defective Viruses Provirus Fusion protein Molecular biology Artificial Gene Fusion Insect Science DNA Viral Chickens |
| Zdroj: | Journal of virology. 75(9) |
| ISSN: | 0022-538X |
| Popis: | Avian leukosis virus subgroup J (ALV-J), the most recent member of the avian retroviruses, is predominantly associated with myeloid leukosis in meat-type chickens. We have previously demonstrated that the acutely transforming virus strain 966, isolated from an ALV-J-induced tumor, transformed peripheral blood monocyte and bone marrow cells in vitro and induced rapid-onset tumors, suggesting transduction of oncogenes (L. N. Payne, A. M. Gillespie, and K. Howes, Avian Dis. 37:438–450, 1993). In order to understand the molecular basis for the rapid transformation and tumor induction, we have determined the complete genomic structure of the provirus of the 966 strain. The sequence of the 966 provirus clone revealed that its genome is closely related to that of HPRS-103 but is defective, with the entire pol and parts of the gag and env genes replaced by a 1,491-bp sequence representing exons 2 and 3 of the c- myc gene. LSTC-IAH30, a stable cell line derived from turkey monocyte cultures transformed by the 966 strain of ALV-J, expressed a 72-kDa Gag-Myc fusion protein. The identification of the myc gene in 966 virus as well as in several other ALV-J-induced tumors suggested that the induction of myeloid tumors by this new subgroup of ALV occurs through mechanisms involving the activation of the c- myc oncogene. |
| Databáze: | OpenAIRE |
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