Cloning and sequence analysis of the TcP2 beta cDNA variants of Trypanosoma cruzi
| Autor: | Hernan Lorenzi, Sergio Ghio, Claudia Ben Dov, Mariano J. Levin, Martin P. Vazquez, Alejandro G. Schijman |
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| Rok vydání: | 1995 |
| Předmět: |
Ribosomal Proteins
DNA Complementary Sequence analysis Trypanosoma cruzi Molecular Sequence Data Biophysics Biochemistry Ribosome Structural Biology Ribosomal protein Complementary DNA Genetics Animals Functional ability Amino Acid Sequence Cloning Molecular Cloning chemistry.chemical_classification biology Base Sequence biology.organism_classification Amino acid chemistry Mutation Sequence Alignment |
| Zdroj: | Biochimica et biophysica acta. 1264(1) |
| ISSN: | 0006-3002 |
| Popis: | P2β acidic ribosomal protein is a relevant antigen in Chagas' disease. cDNA cloning demonstrated that Trypanosoma cruzi expresses at least six types of TcP2β transcripts that differed by point nucleotides substitutions. The distribution and type of mutations seemed to follow the typical structural organization of eukaryotic acidic ribosomal proteins. Most of the synonymous changes clustered in the amino-terminus, suggesting that conservation of this domain was crucial for an equivalent functional ability of each TcP2β variant to bind to the ribosome. Interestingly, most amino acid changes in the central-globular and hinge domains caused polymorphism in putative phosphorylatable sites. |
| Databáze: | OpenAIRE |
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