Patterns of expression of steroidogenic enzymes during the first wave of the ovine estrous cycle as compared to the preovulatory follicle
| Autor: | Baljit Singh, E.T. Bagu, Jaswant Singh, Norman C. Rawlings, R. Duggavathi, D.M.W. Barrett, K.L. Davies, Kyathanahalli S. Janardhan |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Ovulation
endocrine system medicine.medical_specialty 3-Hydroxysteroid Dehydrogenases media_common.quotation_subject Estrous Cycle Follicle Aromatase Endocrinology Ovarian Follicle Food Animals Internal medicine Follicular phase medicine Animals Ovarian follicle media_common Estrous cycle Granulosa Cells Sheep Estradiol urogenital system Chemistry Steroid 17-alpha-Hydroxylase General Medicine Antral follicle Immunohistochemistry medicine.anatomical_structure Theca Theca Cells Female Steroids Animal Science and Zoology Folliculogenesis Estrus Detection |
| Zdroj: | Animal Reproduction Science. 91:345-352 |
| ISSN: | 0378-4320 |
| Popis: | The expression patterns of steroidogenic enzymes in ovarian antral follicles at various stages of growth in a follicular wave have not been reported for sheep. Ovaries were collected from ewes (n=4-5 per group) when the largest follicle(s) of the first wave of the cycle, as determined by ultrasonography, reached (i) 3 mm, (ii) 4 mm, (iii)or =5 mm in diameter or when there was a single (iv) preovulatory follicle in the last wave of the cycle, 12h after estrus detection. The expression pattern of steroidogenic enzymes was quantified using immunohistochemistry and grey-scale densitometry. The expression of CYP19 in the granulosa and 3beta-HSD and CYP17 in the theca increased (P0.01) progressively from 3 toor =5 mm follicles in the first wave of the cycle and was lower (P0.01) in the preovulatory follicle compared toor =5 mm follicles. However, the expression of 3beta-HSD in the granulosa increased (P0.05) from 3 toor =5 mm follicles and was maintained (P0.05) at a high level in the preovulatory follicles. The amount of CYP19 in the granulosa of the growing follicles correlated positively (r=0.5; P0.03) with the concurrent serum estradiol concentrations. We concluded that the expression pattern of steroidogenic enzymes in theca and granulosa of follicles growing in each wave in the ewe, paralleled with serum estradiol concentrations, with the exception that concentrations of 3beta-HSD in granulosa increased continuously from follicles 3mm in diameter to the preovulatory follicle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect