Dissecting myogenin-mediated retinoid X receptor signaling in myogenic differentiation
| Autor: | Munerah Hamed, Jihong Chen, Saadia Khilji, Qiao Li |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Chromatin Immunoprecipitation
Transcriptional regulatory elements Medicine (miscellaneous) Biology Retinoid X receptor MyoD General Biochemistry Genetics and Molecular Biology Article Cell Line Epigenesis Genetic Histones Myoblasts 03 medical and health sciences Mice 0302 clinical medicine Animals Epigenetics Enhancer lcsh:QH301-705.5 Myogenin 030304 developmental biology 0303 health sciences Histone Acetyltransferase p300 Acetylation Cell Differentiation musculoskeletal system Regulatory networks 3. Good health Cell biology Histone Enhancer Elements Genetic Retinoid X Receptors lcsh:Biology (General) Bexarotene Differentiation biology.protein General Agricultural and Biological Sciences C2C12 tissues E1A-Associated p300 Protein 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Communications Biology Communications Biology, Vol 3, Iss 1, Pp 1-12 (2020) |
| ISSN: | 2399-3642 |
| Popis: | Deciphering the molecular mechanisms underpinning myoblast differentiation is a critical step in developing the best strategy to promote muscle regeneration in patients suffering from muscle-related diseases. We have previously established that a rexinoid x receptor (RXR)-selective agonist, bexarotene, enhances the differentiation and fusion of myoblasts through a direct regulation of MyoD expression, coupled with an augmentation of myogenin protein. Here, we found that RXR signaling associates with the distribution of myogenin at poised enhancers and a distinct E-box motif. We also found an association of myogenin with rexinoid-responsive gene expression and identified an epigenetic signature related to histone acetyltransferase p300. Moreover, RXR signaling augments residue-specific histone acetylation at enhancers co-occupied by p300 and myogenin. Thus, genomic distribution of transcriptional regulators is an important designate for identifying novel targets as well as developing therapeutics that modulate epigenetic landscape in a selective manner to promote muscle regeneration. Khilji et al analyse how myogenin distribution changes in differentiating C2C12 myoblasts using ChIP-sequencing. They find that rexinoid x receptor signalling promotes myogenin localisation to poised enhancers and the association of myogenin with p300-mediated histone acetylation, providing insights into epigenetic mechanism of myogenic differentiation. |
| Databáze: | OpenAIRE |
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