The assessment of intracellular magnesium: Different strategies to answer different questions
| Autor: | Stefano Iotti, Emil Malucelli, Giovanna Picone, Concettina Cappadone, Giovanna Farruggia |
|---|---|
| Přispěvatelé: | Picone G., Cappadone C., Farruggia G., Malucelli E., Iotti S. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Metal ions in aqueous solution Clinical Biochemistry Population chemistry.chemical_element 030209 endocrinology & metabolism Mass spectrometry Biochemistry 03 medical and health sciences 0302 clinical medicine Intracellular magnesium Synchrotron-based x-ray microscopy Microscopy Humans Magnesium education Molecular Biology F-AAS education.field_of_study 030109 nutrition & dietetics Fluorescence chemistry Biophysics Inductively coupled plasma Intracellular Plasma mass spectroscopy |
| Popis: | The role of magnesium in cell metabolism is complex and still not completely clarified. Although magnesium has been shown to modulate many phenomena in cells, its intracellular distribution and subcellular compartmentalization have not yet elucidated in detail, mainly as a consequence of the inadequacy of analytical techniques. The method usually employed to quantify total magnesium in cells or tissue are F-AAS or more sensitive techniques as graphite furnace AAS and inductively coupled plasma mass spectroscopy (MS). Thanks to the development of new specific fluorescent dyes, several progresses have been made in the comprehension of the fundamental biological process at the cellular and sub-cellular level. Moreover, the biological function of a chemical element in cells does not only require the determination of its intracellular quantity but also the spatial distribution of its concentration. Most of Mg2+-sensitive fluorescent dyes detect only the free metal ions, precluding the possibility of identifying the total pool of Mg. This review aims at giving an overview on different techniques focusing on two approaches to quantify total Mg in a small cell population or in single cells: i) Indirect Mg detection, label-based methods that represent the best choice to quantify the elemental concentration on a large cell population; ii) direct Mg detection (label-free), Synchrotron-based x-ray microscopy techniques that offer the possibility of achieving a detailed map of the intracellular concentration of a specific chemical element on single cell. |
| Databáze: | OpenAIRE |
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