The Mechanism of Cell Death in Human Cultured Colon Adenocarcinoma Cell Line COLO 201 Induced by .BETA.-D-N-Acetylglucosaminyl-p-Nitrophenol
| Autor: | Ikuko Kakizaki, Masahiko Endo, Akihiko Matsuki, Keiichi Takagaki, Akihiro Munakata, Junko Kukidome |
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| Rok vydání: | 2001 |
| Předmět: |
inorganic chemicals
Programmed cell death Time Factors Cell Culture Techniques Apoptosis Adenocarcinoma Biology General Biochemistry Genetics and Molecular Biology Acetylglucosamine Nitrophenols Tumor Cells Cultured medicine Humans heterocyclic compounds Dose-Response Relationship Drug Molecular Structure General Medicine medicine.disease Molecular biology enzymes and coenzymes (carbohydrates) genomic DNA Biochemistry Cell culture Colonic Neoplasms Toxicity Agarose gel electrophoresis Fetal bovine serum |
| Zdroj: | The Tohoku Journal of Experimental Medicine. 194:23-34 |
| ISSN: | 1349-3329 0040-8727 |
| DOI: | 10.1620/tjem.194.23 |
| Popis: | COLO 201, human colon adenocarcinoma cells were incubated with artificial primers, p-nitrophenyl-glycoside derivatives at 1.0 mmol (mM) in the medium containing 10% fetal bovine serum to detect sugar chain elongation. However, when p-nitrophenyl-beta-N-acetylglucosamine (beta-GlcNAc-PNP) was added, the medium changed color to yellow and the cells were dead. To explain this finding, the cells were incubated with 1.0 mM each of beta-GlcNAc-PNP and 4-methylumbelliferyl-beta-N-acetylglucosamine, then the number of living cells was measured in a time course. In beta-GlcNAc-PNP, the living cells were decreased at 24 hours. The cells were survived with N-acetylglucosamine, whereas in the presence of p-nitrophenol (PNP) the living cells were decreased. It was suggested that PNP released from beta-GlcNAc-PNP induced the cell death. Activity of beta-D-N-acetylglucosaminidase was detected in fetal bovine serum. It was shown that PNP induced the cell death in time-and-dose dependent manner. Genomic DNA from COLO 201 analyzed by agarose gel electrophoresis was fragmentated. PNP analogues were tested for toxicity, and the results suggested that the phenolic OH-group linked to benzene ring and nitro-group linked to the structure in para-form (PNP) was the most effective. |
| Databáze: | OpenAIRE |
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