Polydopamine-Laced Biomimetic Material Stimulation of Bone Marrow Derived Mesenchymal Stem Cells to Promote Osteogenic Effects
| Autor: | Rui Zou, Ching Chang Ko, Dong Joon Lee, Yan Ting Lee, Renie Daniel |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
Indoles Compressive Strength Cell Survival Polymers Surface Properties Science education 0206 medical engineering Bone Marrow Cells 02 engineering and technology Article Fluorescence Rats Sprague-Dawley Calcification Physiologic Biomimetic Materials Osteogenesis Cell Adhesion medicine Animals Bone regeneration neoplasms Cell Proliferation Stem cell transplantation for articular cartilage repair Multidisciplinary Chemistry Skull Mesenchymal stem cell Biomaterial Mesenchymal Stem Cells X-Ray Microtomography HCCS 021001 nanoscience & nanotechnology medicine.disease 020601 biomedical engineering digestive system diseases Cell biology Durapatite medicine.anatomical_structure Medicine Cortical bone Collagen Bone marrow 0210 nano-technology Calcification |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017) |
| ISSN: | 2045-2322 |
| Popis: | A hydroxyapatite-collagen (HC) composite material can mimic composition and ultra-structures of natural bone and provide adequate bioactive material-tissue interactions. Incorporation of dopamine (DA) is one of keys in increasing the mechanical strength of the HC material to approaching that of cortical bone. In this study, the in vitro osteogenic effects of polydopamine-laced hydroxyapatite collagen calcium silicate (HCCS-PDA) were examined by culturing rat mesenchymal stem cells (rMSCs) on HCCS-PDA and HCCS coated plates. HCCS-PDA group demonstrated less cytotoxic from Live/Dead cytotoxic assay and displayed higher cell attachment, proliferation and mineralization than the HCCS group in vitro. For in vivo bone regeneration, HCCS-PDA or HCCS particulates with or without rMSC aggregates were implanted into rat critical-sized calvarial defects (CSD). After 12 weeks, calvarial bone regeneration was evaluated radiographically, histologically, and histomorphometrically. While the majority of new bone formation occurred around the HCCS-PDA particulates with rMSC aggregates, The HCCS-PDA particulates without rMSC aggregates showed limited osteoconductivity. HCCS with or without rMSC aggregates resulted in less bone formation, indicating a prominent role of DA in effective bone regeneration. Therefore, the HCCS-PDA biomaterial with the aid of rMSCs can be used to develop therapeutic strategies in bone tissue engineering with numerable clinical applications. |
| Databáze: | OpenAIRE |
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