The knock‐down of the expression of MdMLO19 reduces susceptibility to powdery mildew (Podosphaera leucotricha) in apple (Malus domestica)

Autor: Richard G. F. Visser, Francesco Salamini, Stefan Martens, Yuling Bai, Angeli Dario, Stefano Pessina, Henk J. Schouten, Riccardo Velasco, Mickael Malnoy
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
Malus
MLO
Arabidopsis thaliana
Plant Science
Plant disease resistance
01 natural sciences
03 medical and health sciences
PBR Biodiversiteit en Genetische Variatie
Powdery mildew
Laboratorium voor Plantenveredeling
RNA interference
Ascomycota
Gene Expression Regulation
Plant
Botany
Gene
Research Articles
Phylogeny
Disease Resistance
Plant Proteins
Genetics
Podosphaera leucotricha
Mildew
biology
food and beverages
biology.organism_classification
PE&RC
Plants
Genetically Modified
Complementation
Settore AGR/07 - GENETICA AGRARIA
Plant Breeding
030104 developmental biology
Malus domestica
Gene Knockdown Techniques
powdery mildew
EPS
Agronomy and Crop Science
PBR Biodiversity and genetic variation
010606 plant biology & botany
Biotechnology
Research Article
Zdroj: Plant Biotechnology Journal
Plant Biotechnology Journal, 14(10), 2033-2044
Plant Biotechnology Journal 14 (2016) 10
ISSN: 1467-7652
1467-7644
Popis: Summary Varieties resistant to powdery mildew (PM; caused by Podosphaera leucotricha) are a major component of sustainable apple production. Resistance can be achieved by knocking‐out susceptibility S‐genes to be singled out among members of the MLO (Mildew Locus O) gene family. Candidates are MLO S‐genes of phylogenetic clade V up‐regulated upon PM inoculation, such as MdMLO11 and 19 (clade V) and MdMLO18 (clade VII). We report the knock‐down through RNA interference of MdMLO11 and 19, as well as the complementation of resistance with MdMLO18 in the Arabidopsis thaliana triple mlo mutant Atmlo2/6/12. The knock‐down of MdMLO19 reduced PM disease severity by 75%, whereas the knock‐down of MdMLO11, alone or in combination with MdMLO19, did not result in any reduction or additional reduction of susceptibility compared with MdMLO19 alone. The test in A. thaliana excluded a role for MdMLO18 in PM susceptibility. Cell wall appositions (papillae) were present in both PM‐resistant and PM‐susceptible plants, but were larger in resistant lines. No obvious negative phenotype was observed in plants with mlo genes knocked down. Apparently, MdMLO19 plays the pivotal role in apple PM susceptibility and its knock‐down induces a very significant level of resistance.
Databáze: OpenAIRE
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