Antigenic relationships between pore proteins of Escherichia coli K12
| Autor: | Ben J. J. Lugtenberg, Nico Overbeeke, Guus Van Scharrenburg |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Salmonella typhimurium
Enterobacter Enterobacter aerogenes medicine.disease_cause Biochemistry Microbiology Organophosphorus Compounds Antigen Bacterial Proteins medicine Escherichia coli Proteus mirabilis Antiserum Antigens Bacterial biology Cell Membrane Membrane Proteins Protein superfamily biology.organism_classification Klebsiella pneumoniae Immunologic Techniques bacteria Bacterial outer membrane Bacterial Outer Membrane Proteins |
| Zdroj: | European journal of biochemistry. 110(1) |
| ISSN: | 0014-2956 |
| Popis: | Antisera were raised against the purified Escherichia coli K12 outer membrane proteins ompA-, ompC- and ompF proteins and protein e. Several immunological methods were used to investigate the specificity of the antisera and the immunological relationship between the major outer membrane proteins. Although the antisera had been raised against highly purified proteins, several of them contained activity against lipopolysaccharide and lipoprotein due to minor impurities in the immunogens. The three general porins ompF protein, ompC protein and protein e were shown to be cross-reactive. Anti-(ompA protein) serum only reacted with the homologous protein. None of these antisera reacted with the phage lambda receptor protein or with protein III. Pore protein preparations isolated from Salmonella typhimurium, Klebsiella aerogenes, Enterobacter cloaceae and Proteus mirabilis were found to be structurally related to the E. coli K12 porins as they reacted with the antisera raised against E. coli K12 porins. |
| Databáze: | OpenAIRE |
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