Biochemical and molecular characterization of lactase producing bacterium isolated from dairy effluent
| Autor: | K. Prakash Narayana Reddy, M. Indira, A. Ranga Rao, K. Abraham Peele, S. Krupanidhi, R. Bharath Kumar, T.C. Venkateswarulu, K. Vidya Prabhakar |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Multidisciplinary biology Strain (chemistry) medicine.medical_treatment Lactase 02 engineering and technology Bacillus subtilis 010501 environmental sciences 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences Enzyme assay Enzyme chemistry medicine biology.protein Zymography Food science lcsh:Science (General) 0210 nano-technology Effluent Bacteria lcsh:Q1-390 0105 earth and related environmental sciences |
| Zdroj: | Journal of King Saud University: Science, Vol 32, Iss 2, Pp 1581-1585 (2020) |
| ISSN: | 1018-3647 |
| Popis: | In the present study, the microbial source for potent lactase producers was explored to supplement the lactase intolerant individuals. Dairy effluent was screened for lactase producing bacteria by conventional microbiological methods. Among the positive isolates, one isolate VUVD001 was found to be a strong producer of lactase enzyme and this strain identified by 16S rDNA analysis. The lactase producing bacterium is identified as Bacillus subtilis by biochemical and 16S rDNA analysis. The VUVD001 strain found to survive in a temperature range of 20–55 °C, pH range of 5–8 and a salt concentration up to 8%. Further, the partially purified enzyme preparation was tested by zymogram analysis for activity testing using x-gal and the enzyme activity of cell free supernatant was estimated to be 15.10 U/ml at 37 °C, 4% NaCl and pH of 7.0. Our finding reports a new isolate Bacillus subtilis VUVD001 strain which can be used as potential strain for commercial production of lactase. Keywords: Lactase, Bacillus subtilis, ortho-nitrophenyl-β-galactoside (ONPG), X-gal, 16S rDNA |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|